INHIBITION OF AMYLIN FIBRIL FORMATION AND PROTECTION OF ISLET β-CELLS BY MEDICINAL PLANTS
Journal Title: International Journal of Pharmacognosy - Year 2015, Vol 2, Issue 5
Abstract
Misfolding of some proteins, their self-assembly into insoluble amyloid structures underlies many diseases, constituting a group of amyloid-related pathology. Fibrillation of peptide hormone of the pancreas, amylin, is considered as one of the causes of death of β-cells in type 2 diabetes. In this work we studied in-vitro protection of islet β-cells from death in the presence of aggregated amylin by ethanol extracts of rose petals (Rosa damascena), melilot (Melilotus officinalis), leaves of grape (Vitis vinifera), sorrel (Rumex confertus) and blackberry (Rubus caesius), and by eleven fractions, isolated from these plants (Coumarin, phenol glycoside, flavonoid and anthracene derivative). A rather low IC50 values in protection of β-cells were estimated for the extracts (0.03-1.5 µg/ml) and their fractions (0.04-3.9 µg/ml). The inhibition of amylin aggregation by several plant extracts and fractions in-vitro was studied using ThT fluorescence method. The IC50 values in deceleration of the peptide aggregation were between 1.76-7.24 µg/ml. A strong correlation (r = 0.99, p=0.0005) was observed between IC50 values in the protection of β-cells and amylin aggregation. Probably, both of these processes are conditioned by prevention of amylin aggregation and decomposition of its preformed aggregates by plant preparations. These results allow recommending frequent use of effective plants by persons at risk group and considering these plants as sources for anti-diabetic agents.
Authors and Affiliations
S. G. Sharoyan et al.
Keywords
Related Articles
- EP ID EP556246
- DOI -
- Views 100
- Downloads 0
EFFECT OF COMMIPHORA MUKUL IN CHRONIC OXAZOLONE INDUCED MOUSE DERMATITIS MODEL
Aim: The objective of this study was to evaluate the effect of Commiphora mukul on psoriasis. Materials and Methods: Six female Balb/C mice per group of which, the first three groups served as untreated, disease controls...
ANTIMICROBIAL ACTIVITY OF SECONDARY METABOLITES FROM PLANTS- A REVIEW
Plants are valuable sources of natural products. Despite the availability of different approaches for the discovery of therapeutically, natural products remain as one of the best reservoirs of new structural types. They...
MEDICINAL EFFECT OF KAMEELA AND AMRAZE JILDIYA (SKIN DISEASES) DESCRIBED IN UNANI SYSTEM OF MEDICINE AND CURRENT RESEARCH-AN OVERVIEW
Kameela or Kamala is a well known Unani drug which is being used as medicament from centuries for the treatment of Amraze Jildiya (skin disease) by Unani Physicians and also mentioned their medicinal efficacy in Unani cl...
POLY HERBAL THERAPY IN OINTMENT BASE FOR WOUND HEALING ACTIVITY IN RATS
In the present study, an indigenous herbal formulation containing the ethanolic extracts of rhizomes of Curcuma longa, fruits of Terminalia chebula, leaves of Azadirachta indica, leaves of Lawsonia inermis and leaves of...
PHYTOCHEMICAL AND PHYSIOCHEMICAL STUDIES OF SOYAMIDA FEBRIFUGA LEAF (MELIACEAE)
The present study mainly focuses on the ethnomedicinal importance of Soymida febrifuga. The selected plant was reported to have wide ethnomedicinal use. The literatures revealed that there is lack of scientific reports o...