INHIBITION OF AMYLIN FIBRIL FORMATION AND PROTECTION OF ISLET β-CELLS BY MEDICINAL PLANTS
Journal Title: International Journal of Pharmacognosy - Year 2015, Vol 2, Issue 5
Abstract
Misfolding of some proteins, their self-assembly into insoluble amyloid structures underlies many diseases, constituting a group of amyloid-related pathology. Fibrillation of peptide hormone of the pancreas, amylin, is considered as one of the causes of death of β-cells in type 2 diabetes. In this work we studied in-vitro protection of islet β-cells from death in the presence of aggregated amylin by ethanol extracts of rose petals (Rosa damascena), melilot (Melilotus officinalis), leaves of grape (Vitis vinifera), sorrel (Rumex confertus) and blackberry (Rubus caesius), and by eleven fractions, isolated from these plants (Coumarin, phenol glycoside, flavonoid and anthracene derivative). A rather low IC50 values in protection of β-cells were estimated for the extracts (0.03-1.5 µg/ml) and their fractions (0.04-3.9 µg/ml). The inhibition of amylin aggregation by several plant extracts and fractions in-vitro was studied using ThT fluorescence method. The IC50 values in deceleration of the peptide aggregation were between 1.76-7.24 µg/ml. A strong correlation (r = 0.99, p=0.0005) was observed between IC50 values in the protection of β-cells and amylin aggregation. Probably, both of these processes are conditioned by prevention of amylin aggregation and decomposition of its preformed aggregates by plant preparations. These results allow recommending frequent use of effective plants by persons at risk group and considering these plants as sources for anti-diabetic agents.
Authors and Affiliations
S. G. Sharoyan et al.
Keywords
Related Articles
- EP ID EP556246
- DOI -
- Views 105
- Downloads 0
AN OVERVIEW OF ANTI-CANCEROUS BEHAVIOR OF VITAMIN E
Imbalance of reactive oxygen species and their body neutralizing mechanisms defines oxidative stress. ROS play a vital role in cancer progression by taking part in DNA damage and induction of inflammatory processes. Stud...
IMPORTANCE OF ANTIMICROBIAL AGENTS FROM PLANTS IN PRESENT SCENARIO: A REVIEW
Since, human existence on earth, plants are being used for the treatment of various diseases. The specific uses of plants are mentioned in the traditional system of medicines like Ayurveda, Siddha, Unani, etc. Now a day’...
FORMULATION AND EVALUATION OF GEL CONTAINING MANGIFERA INDICA LEAVES EXTRACT FOR ANTI-BACTERIAL ACTIVITY
In the present study, an attempt was made to formulate ethosomal gel containing leaf extract of Mangifera indica, and was evaluated for antibacterial activity against gram positive and gram negative organisms. Different...
ANALGESIC ACTIVITY OF CINNAMALDEHYDE PER SE AND IT’S INTERACTION WITH DICLOFENAC SODIUM AND PENTAZOCINE IN SWISS ALBINO MICE.
Cinnamon is one of the best-known spices used as an herbal medicine. Cinnamaldehyde is the most important constituents of cinnamon. The present study was aimed to evaluate the analgesic activity of Cinnamaldehyde per se...
INVESTIGATION ON NOVEL ROLE OF SOLANUM XANTHOCARPUM AND JUNIPERUS COMMUNIS EXTRACT AGAINST CCl4 INDUCED LIVER INJURY
Background: There are very less therapeutic and reliable liver protective drugs in modern medicine to prevent and treat liver injury caused due to drugs. Traditionally Solanum xanthocarpum Schradt. (SX) and Wendl. and Ju...