Inhibition of Biogenic Sulphide Production and Biocorrosion of Carbon Steel by Sulphate-reducing Bacteria Using Ocimum gratissimum Essential Oil
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2016, Vol 10, Issue 2
Abstract
The inhibition effects of Ocimum gratissimum essential oil (OGEO) on biogenic sulphide production and biocorrosion of carbon steel by Sulphate-reducing bacteria (SRB) were investigated in the laboratory. SRB were isolated from injection and produced water from Bonga oilfield in Nigeria. Biogenic sulphide production experiment was setup to monitor sulphide production by SRB. The effects of temperature, pH and salinity on biogenic sulphide production were determined. High sulphide production was observed at temperatures ranging between 20°C – 40°C and pH 7 - 9. Sulphide production decreased as salinity increased. Significant production of sulphide (59-168 mg/l) was detected after 72 hours of incubation of >105 cells/ml SRB consortium under study conditions and the maximum of 293 mg/l was observed after 144 hours at 30°C when pH was 7 and salinity 25 g/l. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of OGEO were determined to be the same at 0.52 mg/ml. Corrosion inhibition efficiency (IE) increased with increase in concentration of extract and decreased with increase in temperature. OGEO gave the highest IE of 69.61% at 20°C, 61.050% at 30°C and 58.01% at 40°C for the 14 days test. For the 28 days test, OGEO gave the highest IE of 71.03% at 20°C, 63.61% at 30°C and 59.41% at 40°C. OGEO+50 ppm D-tyrosine blend gave IE ranging from 89.22 - 92.82% and 84.90 – 92.08% for 14 and 28 days test respectively. Adsorption mechanism of OGEO on carbon steel obeyed the Langmuir isotherm model. Thermodynamic parameters evaluated gave negative values for ΔHads and ΔSads, suggesting that the adsorption reaction is spontaneous at low temperature. OGEO showed activities against SRB isolated from oilfield environment and could be useful for the prevention of biocorrosion of carbon steel structures in this environment.
Authors and Affiliations
O. M. Immanuel, G. O. Abu, H. O. Stanley
Keywords
Related Articles
- EP ID EP351730
- DOI 10.9734/JABB/2016/28786
- Views 57
- Downloads 0
The Amelioration of Cyanide Induced Liver Toxicity with Bentonite Using Wistar Rat as Experimental Model
Aim: The objective of this study was to investigate the ameliorating effect of a natural bentonite found in Nigeria on liver enzymes of Wistar rat fed with cyanide from cassava waste water. The enzymes assayed were alkal...
Gas Chromotography Mass Spectrometric Analysis and Molluscicidal Effect of Crude Saponin from Balanites aegyptiaca (L.) Del.
Crude Saponin extracted from the stem-bark of Balanites aegyptiaca was analysed using gas chromatography-mass spectrometry and also evaluated for its molluscicidal properties against Bulinus globosus and Biomphalaria pfe...
Studies on the Rheological, Microbiological and Sensory Qualities of Weaning Food Formulated from Pearl Millet, Wheat, Cowpea and Groundnut
Weaning foods were formulated in a cereal-legume combination using pearl millet, wheat, cowpea and groundnut. The pearl millet was fermented to produce “Akamu”, the wheat was germinated while the cowpea and groundnut wer...
Evaluation of Latent Infection, Bio-deterioration and Yield of Colocasia esculenta (L.) Schott, Caused by Taro Leaf Blight Disease (Phytophthora colocasiae Raciborski)
Taro leaf blight disease caused by Phytophtora colocasiae is a potential economic disease of the taro crop growing regions of Cameroon and other humid tropical regions of West and Central Africa. Studies were conducted a...
Molecular Based Detection, Validation of a LAMP Assay and Phylogenetic Analysis of Capripoxvirus in Kenya
Aims: To optimize the different available molecular based techniques, validate the Loop-mediated isothermal amplification (LAMP) assay for detection of Capripoxviruses (CaPVs) DNA and deep sequencing to confirm the resul...