Inhibition of Matrix Metalloproteinase-2 Expression by Ethanolic Extracts of Zingiberaceae Rhizomes in Artery Endothelial Cells
Journal Title: Scholars Academic Journal of Pharmacy - Year 2015, Vol 4, Issue 3
Abstract
Atherosclerosis belongs to inflammation-related vascular diseases due to bacterial infection and causes lipid accumulation in artery wall that lead to heart attack and stroke. Matrix metalloproteinase (MMP)-2 and -9 are grouped to zinc-dependent proteases that act in degradation of extracellular matrix in atherosclerotic plaque. Instead of traditional foods, the rhizomes of Zingiberaceae have been empirically used in folk medicines purposes, including natural vascular protection. This study was aimed to test the inhibitory effect of 10 Zingiberaceae rhizomes on the expression of MMP-2 activity in lipopolysaccharide-induced artery endothelial cells by conducting gelatin zymogram. At 1 µg/ml, selected Zingiberaceae rhizomes, i.e. Kaempferia pandurata, Curcuma xanthorrhiza, Alpinia galanga, Zingiberaceae officinale, and Z. officinale Var Rubra, were found to attenuate MMP-2 activity up to 50% compared with LPS-treated cells. In summary, these data suggest that selected Zingiberaceae rhizomes may be applied for potential therapeutics in vascular protection and therapy, in particular atherotherapy. Keywords: Zingiberaceae rhizomes, matrix metalloproteinase-2 activity, atherosclerosis, artery endothelial cells.
Authors and Affiliations
Yanti . , Andre Wiharja, Nikodemus Steven, Surya Fajarianto
Keywords
Related Articles
- EP ID EP377159
- DOI -
- Views 115
- Downloads 0
Protective Effect of Cimetidine, Isosorbide Dinitrate & Vitamin C in Experimental Model of Acute Liver Injury
Abstract: Acute liver injury is a serious state of severe extensive damage of liver tissue caused by various reasons. It is experimentally induced by CCl4 the hepatotoxic agent. Forty healthy rabbits were involved in the...
Improvement of peptide nucleic acid (PNA) synthesis, by use of DIC/Oxyma and microwave heating
Abstract:Peptide nucleic acids (PNAs) are promising in diagnostic applications and of great interest due to their therapeutic potential. PNAs unique chemistry can be readily modified with amino acids or other functional...
Isolation, antibacterial and antifungal evaluation of α-amyrenol from the root extract of Acacia ataxacantha DC
The root-bark of Acacia ataxacantha (Fabaceae) was investigated for medicinal potential. The Phytochemical screening indicated presence of flavonoids, glycosides, saponins, steroids/triterpenes, tannins and alkaloids. Th...
Preclinical Evaluation of Anxiolytic Activity of Aristolochia Indica (Linn) Leaf Extract in Swiss Albino Mice
The light/dark test is based on the aversion of rodents to brightly illuminated areas and on the spontaneous exploratory behaviour of rodents in response to mild stressors, that is, novel environment and light. The test...
Degradation Rate of Ebastine in an Aqueous Solution at pH 1.2 and the Effects of Cyclodextrins
Ebastine (EBA), 4'-tert-butyl-4-(4-(diphenylmethoxy) piperidino) butyrophenone, is a second generation H1-antihistamine. The solubility of EBA is poor in aqueous solutions, but increases in acidic conditions. EBA is degr...