Inhibition of Non-Enzymatic Protein Glycation by Pomegranate (Punica granatum) Whole Fruit and its Components
Journal Title: Journal of Food Chemistry and Nanotechnology - Year 2017, Vol 3, Issue 4
Abstract
The non-enzymatic glycation of proteins, an oxidative-dependent process, initiates the formation of advanced glycation endproducts (AGEs) which leads to protein crosslinking. This study investigates the effect of a phenolic whole pomegranate fruit extract and various parts (aril, peel, and membrane) of pomegranate fruit on the in vitro fructose-mediated glycation of albumin. Compared to apple, whole pomegranate fruit exhibited a much higher total phenolics content and antioxidant potential. Pomegranate fruit decreased glycation by 80% when incubated at a phenolic concentration of 2.5 μg gallic acid equivalents (GAE)/ml; apple, at this phenolic concentration, inhibited protein glycation by only 20%. Pomegranate membrane exhibited the highest total phenolics content and antioxidant potential compared to the pomegranate aril and peel. At 2.5 μg phenolics/ml, the membrane fraction decreased glycation by 85% compared to the aril (42%), and peel (75%). Pomegranate membrane also produced the greatest decrease in glycation when these fractions were incubated at the same antioxidant capacities. These results demonstrate that pomegranate fruit is a potent inhibitor of fructose-mediated albumin glycation when compared to whole apple. The inhibitory activity was concentrated in the pomegranate membrane and is attributed to the presence of punicalagin, which is not found in whole apple.
Authors and Affiliations
Pamela Garner Dorsey, Ronald B. Pegg, Xiaoxi Liao, Phillip Greenspan
Keywords
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- EP ID EP308904
- DOI 10.17756/jfcn.2017-047
- Views 104
- Downloads 0
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