Inhibitory activities of extracts of Rumex dentatus, Commelina benghalensis, Ajuga bracteosa, Ziziphus mauritiana as well as their compounds of gallic acid and emodin against dengue virus
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 4
Abstract
Objective: To investigate the inhibitory effects against dengue virus serotype 2 (DENV-2) by five different fractions (extracted by methanol, ethanol, benzene, chloroform and n-hexane) of Rumex dentatus, Commelina benghalensis, Ajuga bracteosa and Ziziphus mauritiana, as well as their constituents (gallic acid, emodin, and isovanillic acid). Methods: All the samples were tested for cytotoxicity on baby hamster kidney cells by MTT assay and for anti-DENV-2 activity by plaque reduction neutralization assay using two DENV-2 doses (45 and 90 plaqueforming units or PFU). Results: All the samples except isovanillic acid exhibited significant prophylactic effects against DENV-2 infectivity (without cytotoxicity) when administered to cells before infection, but were not effective when given 6 h post-infection. The methanol extract of Rumex dentatus demonstrated the highest antiviral efficacy by inhibiting DENV-2 replication, with IC50 of 0.154 μg/mL and 0.234 μg/mL, when added before infection with 45 and 90 PFU of virus, respectively. Gallic acid also exhibited significant antiviral effects by prophylactic treatment prior to virus adsorption on cells, with IC50 of 0.191 μg/mL and 0.522 μg/ mL at 45 and 90 PFU of DENV-2 infection, respectively. Conclusions: The highly potent activities of the extracts and constituent compounds of these plants against DENV-2 infectivity highlight their potential as targets for further research to identify novel antiviral agents against dengue.
Authors and Affiliations
Riffat Batool, Vincent TK Chow
Keywords
Related Articles
- EP ID EP283768
- DOI 10.4103/1995-7645.231466
- Views 31
- Downloads 0
Acute and chronic toxicity studies of hydromethanol leaf extract of Helianthus annuus Linn. in rats
Objective: To investigate the safety levels of hydromethanol leaf extract of Helianthus annuus Linn. (H. annuus) in rat. Methods: Acute oral toxicity test of hydromethanol leaf extract of H. annuus was conducted through...
The Belt and Road Initiative: Challenges and opportunities in tackling emerging infectious diseases
The majority of emerging infectious disease agents affecting human are RNA viruses that originate from animals. Globalization and climate changes continue to reshape the geographical distribution of humans, animals, vect...
Pharmacological and analytical aspects of artemisinin for malaria: Advances and challenges
Malaria remains a major tropical health burden owing to the development of resistance and decreased sensitivity to the frequently used conventional antimalarial drugs. The drug like artemisinin possesses potent antimalar...
Biological, chemical and pharmacological aspects of Madhuca longifolia
Madhuca longifolia (M. longifolia) is also known as Mahua belonging to the family sapoteace family. M. longifolia is used in traditional and folklore system of medicine widely across India, Nepal, and Sri Lanka for its v...
Protective effects of Balanites aegyptiaca extract, Melatonin and Ursodeoxycholic acid against hepatotoxicity induced by Methotrexate in male rats
Objective: To compare the degree of ameliorative effects of Melatonin (MEL), Ursodeoxycholic acid (UDCA) and Balanites aegyptiaca (BA) against hepatotoxicity induced by MTX for one month. Methods: Eighty adult male rats...