Inhibitory effect of Bis(2-chlorobenzaldehyde)phenyl disulphide diimine on mushroom polyphenol oxidase
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2013, Vol 5, Issue 1
Abstract
The inhibitory effect of Bis(2 chlorobenzaldehyde)phenyldisulphide diimine on both activities of mushroom polyphenol oxidase at (20)°C in a 10 mM phosphate buffer solution (pH 6.8), was studied. L-Dopa and L-Tyrosine were used as substrates of catecholase and cresolase activities, respectively. The results showed that ligand competitively inhibits both activities of the enzyme with inhibition constants (Ki) of 0.5 ± 0.01 and 0.4 ± 0.02?M for catecholase and cresolase respectively, which are good valuses comparing with the reported values for other inhibitors. For further insight a docking study between polyphenol oxidase and ligand was performed. The docking simulation showed that ligand binds in the active site of the enzyme near the Cu atoms.
Authors and Affiliations
Afshin Khorrami *| School of Biological Sciences,Biology of society, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran, afshinkhorramy@gmail.com, Mahdi Alijanianzadeh| School of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran, Ali Moghimi| Department of Chemistry, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
Keywords
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