Inhibitory Effect of Probiotic Bacteria Isolated from Camel Milk on Clinical Strains of Drug-Resistant Helicobacter pylori

Journal Title: Medical Laboratory Journal - Year 2018, Vol 12, Issue 2

Abstract

ABSTRACT Background and Objectives: Helicobacter pylori is the most common cause of gastritis and ulcer worldwide. Treatment of such infections may lead to failure due to drug resistance. This study aimed to investigate the antimicrobial effects of bacteria present in camel milk on the growth of drug-resistant clinical isolates of H. pylori. Methods: In this cross-sectional study, biopsy samples from 75 patients with digestive symptoms were transferred to laboratory in transport medium containing homogeneous compounds. In order to isolate H. pylori, urease-positive biopsies were promptly cultured in brucella agar enriched with defibrinated sheep blood and fetal calf serum. Disk diffusion agar test was used to evaluate antibiotic susceptibility and agar well diffusion method was applied to study the antagonistic effect of probiotics isolated from camel milk on the H. pylori isolates. Results: The frequency of H. pylori isolates was 42.7%. The highest rate of resistance was observed against metronidazole (56.3%). In addition, the rate of resistant to amoxicillin, ciprofloxacin, and clarithromycin and tetracycline was 31.3%, 18.8%, 15.6%, respectively. Lactobacillus plantarum (59.3%) was more frequent than other Lactobacillus species. L. plantarum, Lactobacillus fermentum and Lactobacillus casei showed favorable inhibitory effects against the H. pylori isolates, but L. plantarum (with inhibition zone diameter of 20.3 mm) showed the highest inhibitory effect. Conclusion: Considering the increasing rate of drug resistance and the inhibitory effect of probiotics isolated from milk, health providers recommend that promoting consumption of probiotic food seems beneficial for the general population and those suffering from gastrointestinal disorders. Keywords: Helicobacter pylori, Drug resistance, Camel, milk, Probiotics.

Authors and Affiliations

Keywords

Related Articles

Frequency and Antibiotic Resistance Pattern of Diarrheagenic Escherichia coli (DEC) Strains Isolated from Children Aged Less Than 10 Years

ABSTRACT Background and Objectives: Diarrheagenic Escherichia coli (DEC) pathotypes are important causes of diarrhea among children in developing countries. The objective of this study was to determine the...

Evaluation of Antibiotic Resistance Patterns and Frequency of Carbapenemase-Producing Acinetobacter baumannii Isolates by the Carbacineto NP Test

ABSTRACT Background and Objectives: Acinetobacter baumannii is an opportunistic pathogen associated with nosocomial infections. Treatment of infections caused by this bacterium has become challenging due to inc...

Effect of Eight Weeks Aerobic and Combined Training on Serum Levels of Sirtuin 1 and PGC-1α in Coronary Artery Bypass Graft Patients

Background and objectives: Sirtuin-1 (SIRT1) is a conserved a NAD+-dependent protein deacetylase essential for cellular metabolism and defence against oxidative stress and endothelial dysfunction. This study aimed to co...

Detection of fim, pap, sfa and afa Adhesin-Encoding Operons in Escherichia coli Strains Isolated from Urinary Tract Infections

Background and objectives: Urinary tract infections (UTIs) are one of the most common infectious diseases caused by bacteria. The primary etiologic agent of UTIs is Escherichia coli. Uropathogenic E.coli (UPEC) strains h...

Evaluation of Aflatoxin M1 Residues in Traditional Iranian Cheese (Koupeh Cheese) samples by ELISA

Background and Objectives: Local cheese made from raw milk is one of the most commonly consumed dairy products in the world. Mycotoxin contamination of foodstuff and its transmission to consumers are extremely important...

Download PDF file
  • EP ID EP537665
  • DOI 10.29252/mlj.12.2.20
  • Views 201
  • Downloads 0

How To Cite

(2018). Inhibitory Effect of Probiotic Bacteria Isolated from Camel Milk on Clinical Strains of Drug-Resistant Helicobacter pylori. Medical Laboratory Journal, 12(2), 20-26. https://europub.co.uk/articles/-A-537665