Insect vectors transmitting Phytoplasma to vegetables in Eastern Visayas Philippines
Journal Title: Annals of Tropical Research - Year 2022, Vol 44, Issue 1
Abstract
TPhytoplasma diseases are found affecting vegetables in Eastern Visayas, Philippines. Identifying the insect vectors that transmit phytoplasma in the field is necessary for the management of this disease. This study was conducted to identify the vectors of phytoplasma affecting bitter gourd, loofah, and string beans. Insects associated with these vegetables that had shown phytoplasma symptoms in the field were collected, mass-reared, and the progenies of mass reared insects were used for transmission to healthy host plants. Phytoplasma detection was done through PCR and nest PCR assays. Phytoplasma was positively transmitted to healthy bitter gourd plants by a cicadellid leafhopper, Hishimonus sp. and brown planthopper, Ricania speculum. The ~ 1.25Kb phytoplasma-specific band was amplified in these insects. The phytoplasmas transmitted by the Hishimonus sp. and R. speculum produced two different types of symptoms to the bitter gourd, and rDNA sequence analysis separated them into two clusters which confirmed that the phytoplasmas belong to two different strains. Although the Aphis gossypii and also produced symptoms in bitter gourd and string beans, and the pathogen was detected in the insects, whether they vectored phytoplasma to their respective hosts needs further study, since the symptoms produced were quite different from the ones produced by the cicadellid leafhopper and Ricania speculum.
Authors and Affiliations
Lucia M. Borines, Nickie D. Duero, Joy Adeline C. Nuñez, Rezel Sagarino-Borines, Reny G. Gerona, Sandra McDougall3
Keywords
Related Articles
- EP ID EP755466
- DOI https://doi.org/10.32945/atr4414.2022
- Views 1
- Downloads 0
Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdust
Yeasts have been less frequently reported as xylanase producers compared to bacteria and filamentous fungi. Different cellulosic materials including sawdust are produced on a large scale and these can be used for the pro...
Reformulation of vermicast as organic fertilizer for corn
The increasing demand for healthy and sustainable food production practices has encouraged farmers to switch to the use of organic fertilizers. Information on the nutrient composition of the available organic products is...
Response of Roselle (Hibiscus sabdariffa L.) varieties to fertilizer type and rates in two agro-ecologies of Southwest Nigeria
Roselle is an important traditional vegetable that is drought resistant and tolerant to wide range of soil conditions. It responds well to fertilizer application for optimum productivity. Field trials were conducted simu...
Social impacts of coconet enterprise on smallholder coconut farming family: The case of Panaon Island Farmers Federation Inc. in the Philippines
This study looked into the social impacts of the cocotwine-coconet-making enterprise of the Panaon Island Farmers Federation, Inc. The study followed the constructivist research approach. Results showed that the enterpri...
Indications of enhanced soil ecosystem functions in polyculture reforested grassland
A lot of work has been doneto evaluate the effects of biodiversity on ecological functions in polyculture plantings. But attention is rarely given to soil ecosystem functions such as carbon (C) sequestration and nitrogen...