Inside-out and Standard Vein Grafts, with or without Muscle Filling, in Peripheral Nerve Repair: A Histomorphometric Study
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 13, Issue 1
Abstract
Aim: The aim of this study was to compare two tubulization techniques, inside-out veins and standard veins, both filled with skeletal muscle or not, in sciatic nerve by morphological and histomorphometric study. Methodology: Seventy Wistar rats were divided in 4 experimental groups (IOVNF - inside-out vein with no filling; IOVSM - inside-out vein filled with skeletal muscle; SVNF - standard vein with no filling; and SVSM - standard vein filled with skeletal muscle) and a control group (Sham). The left external jugular vein was sectioned into about 14 mm segments to be used as autologous vein grafts. A 10 mm gap was then created in the sciatic nerve and the vein graft was inserted into the vein with or without filling of the right caudal tibial muscle. The animals were euthanized 12 weeks after surgery. Results: Myelinated and unmyelinated nerve fibers were observed in the histological analyses for all groups, as well as neoformation of the perineurium and intraneural organization of fascicles and blood vessels. In the morphometric analysis of the distal stump, regarding the myelin sheath area, all groups had a significant difference. The IOVNF group had the highest means for fiber, axon and myelin sheath areas. The SVSM group had the lowest means in all features measured, except for the axon area (4.95±1.72 graft; 3.71±0.90 distal stump). Conclusion: These results show that sciatic nerve repair with inside-out veins and no filling (IOVNF) had the best results, in the majority of measured variables, when compared to the other groups.
Authors and Affiliations
Domingos D. Roque, Karina T. Pomini, Rogério L. Buchaim, Daniela V. Buchaim, Jesus C. Andreo, José S. Roque, Antonio C. Rodrigues, Geraldo M. Rosa Júnior, Luis Henrique R. Moraes, Fausto Viterbo
Keywords
Related Articles
- EP ID EP339988
- DOI 10.9734/BJMMR/2016/23294
- Views 83
- Downloads 0
Iron Deficiency among Pregnant Women Attending Antenatal Clinic at the KNUST Hospital, Kumasi, Ghana
Background: Pregnant women constitute a high risk group for iron deficiency due to increased iron requirements for foetal and maternal tissues growth. This study sought to find out the prevalence of iron deficiency among...
Benefits of Incremental Pace Walking in Chronic Obstructive Pulmonary Disease Patients
Background: Exercise training in chronic obstructive pulmonary diseases (COPD) patients can be challenging due to exertional dyspnea. It has been suggested that gradual exercise training may result in improved exercise c...
Prostate Cancer Incidence in Calabar - Nigeria
Aim: The aim of this study is to determine the incidence of prostate cancer in men living in Calabar- Nigeria, which has not been determined up till now. Study Design: A trend analysis of prostate cancer cases in Calabar...
Anterior Chest Wall Tuberculosis Involving Lungs, Pleura and Lymph Nodes in an Immunocompetent Patient: A Case Report
Aims: The chest wall tuberculosis accounts for 1-5% of all cases of musculoskeletal tuberculosis. Here we are presenting a rare case of anterior chest wall tuberculosis in an immunocompetent patient. Presentation of Case...
Physical Fitness and Prevalence of Musculoskeletal Pain amongst Dentists and Dental Students in Mumbai City: A Questionnaire-based, Cross-sectional Study
Introduction: Musculoskeletal pain is one of the most common complaints amongst dental healthcare professionals by virtue of the precision of work demanded from them, which leads to unnatural, static postures and repetit...