Insight into the Possible Association between Gene Polymorphism of IFN-γ and IL-22 with Psoriasis in Punjab, India
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 10
Abstract
ABSTRACT Introduction: IFN-γ and IL-22 are Th1/Th17 cytokine which have been shown to play important role in the development of autoimmune disorder like psoriasis. Aim: To identify the possible association of IFN-γ and IL-22 Single Nucleotide Polymorphisms (SNPs) with psoriasis as well as to find correlation between serum level of IL-22 and IFN-γ with severity of psoriasis. Materials and Methods: A total of 168 psoriasis patients and 152 healthy controls were included in the study. A 3 ml of blood sample was collected from each psoriasis patients and healthy control for further analysis of SNP and serum level of cytokine. A part of blood sample was used for isolation of DNA for gene polymorphism study in psoriasis patients. Gene polymorphism of IL-22 (rs1179251 and rs2227513) and IFN-γ (rs2430561, rs2069709) were studied by ARMS PCR method whereas serum level of IL-22 and IFN-γ was analysed using commercially available ELISA kits. Odds Ratio (OR) with 95% Confidence Interval (95% CI) was calculated to assess the relative risk of psoriasis for various genetic variants. Results: Serum level of IL-22 and IFN-γ was found to be (74.4±24.1 and 96.7±31.7 pg/ml respectively) significantly high (p<0.001), and associated with PASI score in psoriasis patients. In genotype analysis, a significant association was found between gene polymorphism of IFN-γ (rs2430561 and rs2069709) and IL-22 (rs1179251 and rs2227513) with psoriasis. Conclusion: These results suggest that polymorphism of IFN-γ and IL-22 genes can be responsible for elevated serum level of IFN-γ and IL-22 cytokines. As these cytokines play key role in pathogenesis of psoriasis thus in conclusion gene polymorphism of IFN-γ and IL-22 may be responsible factor for increased susceptibility to psoriasis in north Indian population, Punjab, India.
Authors and Affiliations
Arun Kumar Rawat, Aadil Wani, Tahseena Akhtar, Parneet Kaur, Tarun Narang, Dimple Chopra, Tej Bali Singh, Rajinder Kaur
Keywords
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- EP ID EP525986
- DOI 10.7860/JCDR/2018/36381.12154
- Views 49
- Downloads 0
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