Insilico Comprehension of Stop Codon Readthrough in Human Viruses
Journal Title: International Journal of Trend in Scientific Research and Development - Year 2020, Vol 4, Issue 4
Abstract
Readthrough is an event in which stop codon is misread, resulting in elongation of polypeptides. Stop codon suppression or termination codon readthrough, is a mechanism of expression of many disorder proteins. Many important cellular functions are carried out by way of the Readthrough process. This could alter the gene function which thereon produces either destructive or constructive effects. Hence, this study aims to diagnose this recoding mechanism in certain selected humans infecting pathogenic viruses through insilico approach. For this target, the 3'UnTranslated Regions of the selected viruses were retrieved from the Genbank database. Each of these 3'UTRs were translated into all their reading frames. Motif search using Interproscan in each of the frames, followed by homology search using BLASTX, and were achieved to identify stop codon readthrough candidates in each of the selected viruses. Finally, the secondary structure of RNA was predicted using RNAFold web server to ensure the stability of the RNA. The 3'UTRs from Aichi Virus 1, Cosa Virus A, Dengue Virus 1, Duvenhage Lyssavirus, Enterovirus A, HepatitisGB Virus B, Human Cosavirus A, Human Pegivirus 2, Langat Virus, Parechovirus A, WestNile Virus and Zika Virus were retrieved. A total of 48 motifs were identified in different reading frames of 3'UTR of the selected viruses. BlastX search recognized 9 homologs in the reading frames of 3'UTR. The secondary structure analysis and search of motifs and homologs resulted in the confirmation of 5 candidates with strong evidence for the readthrough event. These candidates showed homology with proteins of prime importance such as Imidazole glycerol Phosphate synthase protein, 50S ribosomal protein L27, DNA replication, and repair protein, replication origin binding protein, and adenosine deaminase. Hence, we proved that the 3'untranslated regions would undergo translation. This strongly suggests that many such readthrough events are to be determined to exactly unravel the pathogenicity behind Viruses. To design anti viral drugs to impede this viral machinery, it is essential to analyse their 3'UTR regions. Arockiyajainmary M | Balaji S | Sivashankari Selvarajan "Insilico Comprehension of Stop Codon Readthrough in Human Viruses" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020, URL: https://www.ijtsrd.com/papers/ijtsrd31550.pdf
Authors and Affiliations
Arockiyajainmary M | Balaji S | Sivashankari Selvarajan
Keywords
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