INSULIN- LIKE ACTIONS OF THIOPROPANOL DISULFIDE IN ISOLATED ALLOXAN DIABETIC RAT LIVER
Journal Title: Journal of Evidence Based Medicine and Healthcare - Year 2019, Vol 6, Issue 8
Abstract
BACKGROUND Major actions of insulin include glucose uptake, utilization and storage as well as protein and fat synthesis. Diabetes mellitus (DM) is a metabolic disease mainly results due to insulin deficiency or failure in insulin action. Plant extracts have been used as medicine for DM since ancient times. The allium plants’ active principles are found to be mainly disulfides and sulfoxides and their hypoglycaemic values are well established. Our earlier studies with a low molecular weight thiol, 3-mercapto-1-propanol (thiopropanol), have shown insulin-like actions in isolated liver tissues of alloxan diabetic rats. A study was undertaken to assess the insulin-like actions of thiopropanol disulfide (TPDS) prepared from thiopropanol. MATERIALS AND METHODS Male Swiss albino rats weighing 200-250 g were employed. Alloxan monohydrate was used to induce diabetes. Optimum dosage of TPDS was determined. Six normal and six diabetic rats were sacrificed on the 31st day of the study. Extracted liver tissues were divided into three groups namely- normal, control alloxan diabetic and TPDS-exposed alloxan diabetic groups. Levels of liver tissue glucose, glycogen, lactate, pyruvate, total thiol groups, alanine, total amino acid nitrogen (AAN) as well as activities of hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), alanine transaminase (ALT) and aspartate transaminase (AST) were estimated. Per hour glucose utilization, lactate production, pyruvate production and amino acid utilization were calculated. RESULTS The results showed significant increase (p<0.001) in glucose utilization, lactate production, pyruvate production, total thiol groups, AAN, alanine, HK activity and G6PD activity, and significant decrease (p<0.001) in activities of ALT and AST in TPDSexposed alloxan diabetic liver slices as compared to control alloxan diabetic liver slices. CONCLUSION TPDS undergoes sulfhydryl cleavage like any other disulfide to produce its corresponding thiol component which is responsible for the increased glucose utilization.
Authors and Affiliations
Divya Dattaprasad, Vickram Vickram, Kashinath Rattihalli Thirumalarao
Keywords
Related Articles
- EP ID EP611029
- DOI 10.18410/jebmh/2019/121
- Views 71
- Downloads 0
EASE OF ACCESS TO THE SUBARACHNOID SPACE AND ITS ASSOCIATION WITH SPINOUS PROCESS DIMENSIONS, IN PATIENTS UNDERGOING SPINAL ANAESTHESIA
BACKGROUND Spinal anaesthesia is a procedure commonly performed by the anaesthesiologist. Various reasons can lead to a difficulty in performing a lumbar puncture, leading to multiple attempts, thereby increasing chances...
A STUDY ON KNOWLEDGE AND PRACTICE OF IMMUNIZATION SERVICES AMONG AUXILIARY NURSE MIDWIVES OF SUBCENTRE AT CHIRANG DISTRICT, ASSAM
INTRODUCTION Immunization is one of the cost effective intervention for prevention of major illness in child. Only availability of vaccines is not sufficient, there are various other factors which influences the effectiv...
INTRASPINAL HYDATID CYST- RARE CASE REPORT
PRESENTATION OF CASE A 20-year-old male presented to us with complaints of urinary incontinence and right lower limb weakness. On examination the patient had continuous urinary dribbling with lower motor neuron type of b...
A STUDY ON MORBIDITY PROFILE OF GERIATRIC PATIENTS ADMITTED IN INTERNAL MEDICINE DEPARTMENT OF TERTIARY CARE HOSPITAL IN SOUTH ASSAM, INDIA
BACKGROUND The age structure of the population in the developed countries has so evolved that the numbers of old people is continually on the increase. Worldwide by 2025, the number of elderly people is expected to rise...
WOULD ABORTION SERVICES IN INDIA BENEFIT FROM LESSER HURDLES?
BACKGROUND The aim of the study was to evaluate if service providers are unsupportive of second trimester abortions in young girls. MATERIALS AND METHODS Questionnaires were answered by 766 obstetricians from 24 obstetri...