Insulin-like growth factor - I (IGF-I) complexes in human saliva.

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Insulin-like growth factor - I (IGF-I) complexes in human saliva.

Journal Title: Journal of Stomatology (Czasopismo Stomatologiczne) - Year 2012, Vol 65, Issue 2

Abstract

Introduction. Until now, various biologically active peptides have been detected in saliva, which include, among others, insulin-like growth factors: IGF-I and IGF-II. They circulate in plasma in the form of complexes with binding proteins – IGFBP. Aim of the study. To determine expression and concentration of IGF-I with its complexes in saliva of healthy individuals in different age groups. Material and methods. The study material included samples of resting mixed saliva obtained from 70 healthy volunteers divided into 7 groups according to age and gender. Both Western immunoblot and ELISA tests were used for the qualitative and quantitative evaluation of complexes and binding proteins of IGF-I. Results. Human saliva contains IGF-I predominantly in the form of high molecular mass complexes. The level of free IGF-I is very low and depends on gender and age. Conclusion. Presumably, this factor has the ability of forming complexes with binding proteins, most likely with a binding protein 3 ( IGFBP-3) or with a binding protein 5 (IGFBP-5). A single band of high molecular weight of about 150 kDa, possibly corresponding to IGF/binding proteins/acid labile subunit complex was shown with the use of Western immunoblot method under non-reducing conditions.

Authors and Affiliations

Ewelina Dargiewicz, Małgorzata Wolańska

Keywords

Saliva IGF-I IGFBP-3

Report on the International Scientific Conference of the Periodontological Section of the polish Dental Society Advance in the diagnostic and treatment of diseases of the periodontium and oral mucosa.

EP ID EP57622
DOI -
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