Insulin resistance is associated with increased IRS-1 serine phosphorylation in fat fed rats
Journal Title: Medpulse International Journal of Biochemistry - Year 2018, Vol 8, Issue 3
Abstract
Insulin resistance is an important factor in the pathogenesis of type 2 diabetes. Increased intake of dietary fat induces insulin resistance in both humans and experimental animals. The molecular basis that leads to high fat diet induced insulin resistance are not clearly identified. In the present study, we fed the control group rats with rodent chow and experimental group rats with a high fat diet for the period of nine weeks. Nine weeks of fat feeding to the rats induced insulin resistance when compared to the chow diet fed control rats. Insulin signaling study in skeletal muscle of fat fed rats showed normal insulin stimulated tyrosine phosphorylation of insulin receptor. But we found a significant increase in the serine phosphorylation of IRS-1 and a decreased insulin stimulated tyrosine phosphorylation of IRS-1 in the high fat fed rats compared to the control rats. These results indicates, increased serine phosphorylation of IRS-1 decreases the insulin stimulated IRS-1 tyrosine phosphorylation and leads to insulin resistance in fat fed rats. The association of increased IRS-1 serine phosphorylation with insulin resistance may help us in the understanding of mechanisms of insulin resistance in obesity/dyslipidemia induced diabetes and provide new therapeutic targets for the management of type 2 diabetes
Authors and Affiliations
Vinayagamoorthi R, Selvakumar C, Zachariah Bobby
Keywords
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- EP ID EP442178
- DOI 10.26611/10028234
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