Intact parasite-antigen ELISA test: a new dimension for serodiagnosis of Amoebiasis
Journal Title: Journal of Applied and Natural Science - Year 2018, Vol 10, Issue 3
Abstract
An Intact Parasite Antigen ELISA (IPA-ELISA) has been developed for detection of circulating antibodies against Entamoeba. histolytica. Axenically grown trophozoites of E.histolytica (NIH-200) after glutaraldehyde treatment, are used as Intact Parasite Antigen (IPA) as well biological active and imperishable base. Antigen over the surface of treated cells were allowed to interact with the antibody molecules of the test sera. The techniques of Plate, Dot-and IPA-ELISA are compared of their merits for clinical and epidemiological survey of amoebiasis. IPA-ELISA was found to be more sensitive (96.8%) and specific (92.3%) in detecting circulating antibodies compared to Plate-ELISA (sensitivity 90.5%, specificity 84.6%) and Do-ELISA (sensitivity 92.1%, specificity 86.1%). The entire IPA-ELISA test could be completed within 3 hours. Costwise IPA-ELISA is several times lesser than Dot-and Plate-ELISA. This test is most suitable for field and clinical trials for suspected cases of amoebiasis with no skilled hand required for diagnosis.
Authors and Affiliations
Archana Mishra, Ajay Kumar Sharma, Vandana Tewari
Keywords
Related Articles
- EP ID EP378148
- DOI 10.31018/jans.v10i3.1835
- Views 69
- Downloads 0
Influence of agronomic practices on severity of late blight of potato (Phytophthora infestans)
An experiment was carried out to find out the influence of such agronomic practices on severity of the disease and to seek a proper cultural practice for management late blight of potato (Phytophthora infestans). The exp...
Analysis of bioactive phytochemicals and evaluation of antioxidant activity of a medicinal plant, Boerhaavia diffusa L.
Boerhaavia diffusa L. (Family: Nyctaginaceae) commonly known as Punarnava is an herbaceous, spreading vine widely distributed in the tropical and subtropical regions in the world. The plants are a rich source of vitamins...
Studies on dyeing and bacterial resistance of Dacryodes macrophylla extract on woollen fabric
The dyeing of wool fabric using a natural dye extracted from Dacryodes macrophylla has been studied using both conventional as well as ultrasonic dyeing methods. Natural dye was extracted using water as solvent by Soxhle...
Constraints analysis in adoption of best farm practices towards sequestration of greenhouse gases
The aim of this paper is to find out the major losses due to emission of green house gases (GHGs) and investigate the major constraints that are responsible for non-adoption of best farming practices to minimize the emis...
Isolation, characterization and antibiotic susceptibility profile of Staphylococcus aureus from raw milk samples in Nagpur district, India
The study was carried out from July 2010 to June 2011. The aim of the present investigation was to isolate Staphylococcus aureus from raw milk obtained from cow from different parts of Nagpur district, India and to deter...