Intestinal microbiota of ornamental fish Carassius auratus
Journal Title: Scientific Journal of Animal Science - Year 2016, Vol 5, Issue 2
Abstract
Intestinal microbiota is an essential component for any organism life, because it directly affects nutrient assimilation, growth and health processes. However, in many animals and in case of fish is unknown how Intestinal microbiota is formed, which species are dominant and which one has capacity probiotic for use in aquaculture. The goal of this study was to establish the bacterial load that dominates the back intestine of Carassius auratus.A batch of 200 healthyjuvenilefishes were obtained from an ornamental fish farm in Mexico City. The fish were maintained in two culture beakers of 100 L during 15 day to acclimatization to ensure any sign of injury disease. To extract the back of gastrointestinal tract, which was rinse several times with distilled water for food and feces residues elimination. The sample was inoculated in 9 mL of sterile saline solution and from this, three dilutions in a 1:10 relation were made, inoculating 0.1 mL from each dilution in agar plates MRS, BHI and TCBS, and were incubated at 27ºC for 24 hours. Subsequently a colony forming units (CFU mL-1) count was made, with the help of a Quebec counter type. Colonies were purified through successive inoculations. The molecular identification was made out by sequencing the gene RNAr 16S using Wizard Genomic. Molecular identification showed that the back region of the intestinal tract of C. auratus was dominated by phylum Proteobacteria and Firmicutes represented by the genus Bacillus, Vibrio, Vagococcus, Brevibacillus, Aeromonas, Pseudomonas, Shewanella, Enterococcus, Paenibacillus and Morganella.In relation to bacterial abundance by specie it was established that Bacillus sp. and Pseudomonas stutzeriwere the most abundant reaching CFU mL-1 counts of 210 and 167 respectively, followed byPaenibacillus lactis with 103 CFU mL-1 and Bacillus cereus with 100 CFU mL-1 while Enterococcus eurekensiswas the least abundant with an average value of 6 CFU mL-1.
Authors and Affiliations
Dafne Itzel Orozco Rojas| Departamento El Hombre y su Ambiente, Calzada del Universidad Autónoma Metropolitana-Xochimilco, Hueso No. 1100, Col. Villa Quietud, 04960 México DF, México., María del Carmen Monroy-Dosta*| Departamento El Hombre y su Ambiente, Calzada del Universidad Autónoma Metropolitana-Xochimilco, Hueso No. 1100, Col. Villa Quietud, 04960 México DF, México., Jorge Castro Mejía| Departamento El Hombre y su Ambiente, Calzada del Universidad Autónoma Metropolitana-Xochimilco, Hueso No. 1100, Col. Villa Quietud, 04960 México DF, México., Aida Hamdan Partida| Departamento Atención a la Salud, Calzada del Universidad Autónoma Metropolitana-Xochimilco, Hueso No. 1100, Col. Villa Quietud, 04960 México DF, México., Alberto Ramírez Torrez| Departamento Atención a la Salud, Calzada del Universidad Autónoma Metropolitana-Xochimilco, Hueso No. 1100, Col. Villa Quietud, 04960 México DF, México.
Keywords
Related Articles
- EP ID EP834
- DOI 10.14196/sjas.v5i2.2107
- Views 424
- Downloads 20
Effects of grass and concentrate feed on growth performances of rumbi lamb and meat fat composition
This study aims to evaluate and compare the effect of grass and standard feed on lamb zoo technical parameters and meat fatty acid (FA) composition. 36 lambs of Rumbi breed were used in this trial, of which 24 lambs we...
Physical characteristics, nutritive value and preference of Zebu heifers for ensiled mixtures of corn cob, cassava peel
In order to improve the feeding quality of corn cob and cassava peel for zebu cattle, a mixture of corn cob-cassava peel was ensiled with 0, 1, 2, and 3% of urea. Physical characteristics, chemical composition and in vit...
Nitrogen balance of lactating West African dwarf ewe fed Mexican sunflower leaf meal based diets
Fifteen days prior to weaning, sixteen West African dwarf (WAD) ewes (Initial BW 19.13±1.53kg) on a basal diet of Panicum maximum were allotted into 4 treatment groups A, B, C and D of 4 replicates each. The mexican su...
The egg production performance of three layer strains kept under intensive system in the hot and humid tropics
The egg production industry in Ghana is bereft with many challenges; prominent among them is the appropriate layer strain to use to achieve optimum production. It was against this background that a study was carried ou...
Nutrients digestibility of rice milling waste ( RMW ) and soyabean meal residue (S MR ) combination fed to Yankasa ram l
This experiment was conducted at Bayero University Kano, to determine the Utilization and digestibility of Soyabean meal Residue (SMR) and Rice Milling Waste by Yankasa ram lambs. The 20 animals were fed with a complete...