INTRACELLULAR TOXOPLASMA GONDII ELIMINATION IN PRESENCE OF JcTI-I, A PROTEASE INHIBITOR FROM JATROPHA CURCAS SEED CAKE
Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2017, Vol 4, Issue 6
Abstract
Toxoplasma gondii is the agent of toxoplasmosis, a widespread disease of medical and veterinary importance where the parasites infect all eukaryotic cells of vertebrate animals. There is no effective and low toxic treatment to eliminate the intracellular parasites, perpetuating the infection. The Jatropha species, including Jatropha curcas, acquired high economic importance as a source of high-quality biofuel and medicinal plant. Nevertheless, the anti-parasite activity of the Jatropha species has not been reported to date. In this study, the effects of a protease inhibitor from Jatropha curcas seed cake, named JcTI-I, on extra- and intracellular tachyzoites of T. gondii were assessed in vitro. Extracellular tachyzoites treated before invasion had morphological modifications but did not altered the establishment of infection. The incubation of infected host cells with 5, 10, 50, and 100 μM JcTI-I for 24 h decreased the intracellular parasite number (IC50 = 4.57 μM), without causing a significant reduction in the infected host cell population. Moreover, JcTI-I led ultrastructural alterations on intracellular tachyzoites as assessed by light and transmission electron microscopy. In summary, JcTI-I is a promising candidate for drug development to treat toxoplasmosis and other intracellular parasites.
Authors and Affiliations
Laís Pessanha de Carvalho
Keywords
Related Articles
- EP ID EP630353
- DOI -
- Views 136
- Downloads 0
GAMMA GLUTAMYL TRANSFERASE AS PREDICTIVE MARKER OF CAROTID INTIMA MEDIA THICKNESS IN TYPE 2 DIABETIC SUBJECTS
Objective: To analyze the Gamma Glutamyl Transferase (ϒ-GT) as predictive marker of carotid intima media thickness (CIMT) in type 2 Diabetic subjects. Study Design: Case control study. Place & Duration: Department of Med...
EFFECT OF VASCULAR SMOOTH MUSCLE CELL PROLIFERATION OF URSOLIC ACID
Ursolic acid is present in many plants, such as Mirabilis jalapa, as well as in many fruits and herbs. Ursolic acid has been reported to exhibit multiple biological effects including anticancer, antioxidant, anti-inflamm...
PHYTOCHEMICAL STUDY AND EVALUATION OF ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF VITEX DONIANA SWEET (VERBENACEAE)
Owing to difficulties encountered in the use of synthetic antioxidants, research is moving more and more towards natural antioxidants particularly those from plants. It is in this context that we were interested Vitex do...
SAFETY EVALUATION OF TRAMAL PLUS® (TRAMADOL HYDROCHLORIDE/ACETAMINOPHEN) FOR PAIN MANAGEMENT IN PAKISTANI REAL WORLD PRACTICE (STEP STUDY)
Objective: The objectives of the study were to evaluate adverse effects mainly Central Nervous System (CNS) and Gastrointestinal (GI) in Pakistani subjects by using Tramal Plus® (Tramadol 37.5mg/Paracetamol 325mg) during...
SERUM HOMOCYSTEINE AND VITAMIN B12 IN METFORMIN TREATED TYPE 2 DIABETIC SUBJECTS
Objective: To analyze serum homocysteine and vitamin B12 in type 2 diabetics taking metformin therapy. Study Design: Cross sectional study design. Study setting & Duration: Department of Medicine, Liaquat University Hosp...