Investigate of extractions and chromatographyc conditions gydazepam’s aminobenzophenones
Journal Title: Фармацевтичний журнал - Year 2014, Vol 4, Issue 4
Abstract
Detection aminobenzophenones, after hydrolysis of a biological tissue, represents one of stages of screening both the derivatives of 1,4-benzodiazepine, and the research on «general unknown». One of factors of successful detection and identification is the knowledge of physical and chemical properties of required substances. The purpose of this work, to study influence of the nature of extragent and enviroment acidity on extraction gydazepam’s aminobenzophenones from hydrolysates and also to find out their chromatographic behaviour in the conditions of thin-layer chromatography. Model solutions gydazepam’s aminobenzophenones in buffer solutions and liver hydrolysate were objects of research. A quantitative assessment made a gas-liquid chromatography method. The chromatographic behaviour was studied in twenty systems for thin-layer chromatography. In findings of investigation it is discovered that extraction of the most typical aminobenzophenone of gydazepam, considerably influences рН, whereas extraction of other two gydazepam’s aminobenzophenones, рН practically does not influence. Studying of the nature of extragent has shown possibility of application of all most widespread solvents in laboratory practice. The exception is only by non-polar hexane and heptane. Chromatographic fractionation gydazepam’s aminobenzophenones in various systems has shown significant distinctions between them in chromatographic mobility. Also discovered that on their identification influence aminobenzophenones from other derivatives 1,4-benzodiazepinе. It is discovered that optimal conditions of extraction gydazepam’s aminobenzophenones are рН ≤ 5, extragent n-chlorbutan (and also dichloromethane and chloroform). In the conditions of the classical schema of screening derivatives 1.4-benzodiazepine, difficulties in detection and identification gydazepam’s aminobenzophenones can arise a thin-layer chromatography method.
Authors and Affiliations
M. A. Savchenko, G. P. Petyunin
Keywords
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