Investigate the antimicrobial effect of chicory leaf extract with different solvents on Staphylococcus aureus and Escherichia coli
Journal Title: International Journal of Biosciences (IJB) - Year 2014, Vol 5, Issue 9
Abstract
Recent studies have found some of the important constituents in chicory such as caffeic acid derivatives, fructooligosaccharides, flavonoids,inulin, and polyphe-nol. Cichorium intybus L. is a widespread weed with antibacterial effect. In other reports on the antimicrobial activity of C.intybus, the crude aqueous and organic seed extracts were found to be active against four pathogenic microorganisms, namely,Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans, and root extracts had pronounced effects on Bacillus subtilis, S.aureus, Salmonella typhi, Micrococcus luteus, and E. coli.The main goal of our research is to investigate the antimicrobial effect of chicory extract with different solvents on Staphylococcus aureus and Escherichia coli. In our research the antimicrobial activity of the C. intybus leaves extract and its different fractions was determined by the disc diffusion method. In this study, 67%ethanol, 67%acetone and 67% hexane were used for extraction. Three Blank discs placed in each extract dilution tube and after30 minutes, disks were removed from the tubes and placed in an incubator. For control group we were used Blank discs with solvents that they were dried in same method. We were designed 4 disks per culture plate for each dilution thatone of them was control disk. Staph-aureus and E-coli antibiograms with different solvents had no significant difference between groups and no inhibition zone observed around different extract disks and control disks on Mueller-Hinton agar culture. In our research we couldn’t find any chicory leaves extract antibacterial effect against S.aureus and E. coli
Authors and Affiliations
Masood Khakzadihe, Hadi Eslami, Mohamad Rasoulifard, Hossein Zadeh, Mohammad Behboudi
Keywords
Related Articles
- EP ID EP100094
- DOI http://dx.doi.org/10.12692/ijb/5.9.399-8
- Views 185
- Downloads 0
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Investigate the antimicrobial effect of chicory leaf extract with different solvents on Staphylococcus aureus and Escherichia coli
Recent studies have found some of the important constituents in chicory such as caffeic acid derivatives, fructooligosaccharides, flavonoids,inulin, and polyphe-nol. Cichorium intybus L. is a widespread weed with antibac...