Investigating Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Hospital Infections in Ahvaz
Journal Title: International Journal of Medical Laboratory - Year 2015, Vol 2, Issue 3
Abstract
Background and Aims: The indiscriminate use of antibiotics can lead to antibiotic resistance in the treatment of infections caused by bacteria such as Pseudomonas aeruginosa. The presence of integrons in Pseudomonas is clearly associated with multidrug resistances. Therefore, this study aimed at tracking class I, II and III integrons of antibiotic-resistant isolates of Pseudomonas aeruginosa that were isolated from nosocomial infection. Materials and Methods: In this study, 51 isolates of Pseudomonas aeruginosa were collected from different wards of Imam Khomeini hospital of Ahvaz since October of 2014 until March of 2015. After identification test and antibigram, coding genes of antibiotic resistance and class I, II and III integrons were detected by polymerase chain reaction (PCR) method. Results: Tetracycline revealed the most resistance with 84% frequency in discreted isolates. In the encoding antibiotic resistance genes with a frequency of 94% was the most common blaTEM. Class I integron had 92% prevalence, class II Integron showed 52% prevalence and class III Integron demonstrated 17% prevalence. Conclusions: In Pseudomonas aeruginosa, class I integron was more prevalent than other integrons and the integrase gene was probably one of the causes of multiple antibiotic resistance in this bacteria. Moreover, frequency of integron III was reported 17%.
Authors and Affiliations
Parvin Hosseini Pour, Hassan Momtaz, Amir Arsalan Serajyan, Elahe Tajbakhsh
Keywords
Related Articles
- EP ID EP212140
- DOI -
- Views 141
- Downloads 0
Effects of Methylglyoxal and Aspirin on In Vitro Coagulation and Clot Permeability
Backgrounds and Aims: Methylglyoxal (MGO) is an –α, β dicarbonyl aldehyde inevitably produced from triose-phosphate intermediates of glycolysis, and amino acid. Increased MGO in blood leads to alterations in coagulation,...
Determination of Helicobacter pylori Antigen in Stool Samples and Comparison with Rapid Urease Test in Patients Suspected of Helicobacter Infection
Background and Aims: Helicobacter pylori (H. Pylori) is the microorganism that infects nearly half of word's population. There are several invasive and non-invasive methods for diagnosis of infection. The main objective...
Transforming Growth Factor-β1 Preserves Bovine Nasal Cartilage against Degradation Induced by Interleukin-1α in Explant Culture
Background and Aims: Chondrocytes and their differentiation play a central role in joint diseases. Effect of the transforming growth factor (TGF)-β1 on chondrocyte characteristics and differentiation is not clearly under...
Evaluation of c-Myc mRNA Expression Level in Benign Prostatic Hyperplasia and Prostatic Adenocarcinoma Tissues and Its Correlation with Clinicopathological Characteristics
Background and Aims: Prostate cancer (PCa) is one of the most common cancers among men in Iran. Since changes in the regulation of proto-oncogenes expression are the main causes of most human cancers, including PCa, eval...
The Toxicity of Thorium Oxide Nanoparticles Conjugated with Folic acid and Antibody on the Mononuclear Cells from Patients with Chronic lymphoid Leukemia and Normal Persons
Background and Aims: The aim of this study was to synthesize antibody-conjugated radioactive(thorium oxide) nanoparticles (ACRNPs) and folic acid-conjugated radioactive nanoparticles (FACRNPs). Materials and Methods: Af...