Investigating Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Hospital Infections in Ahvaz
Journal Title: International Journal of Medical Laboratory - Year 2015, Vol 2, Issue 3
Abstract
Background and Aims: The indiscriminate use of antibiotics can lead to antibiotic resistance in the treatment of infections caused by bacteria such as Pseudomonas aeruginosa. The presence of integrons in Pseudomonas is clearly associated with multidrug resistances. Therefore, this study aimed at tracking class I, II and III integrons of antibiotic-resistant isolates of Pseudomonas aeruginosa that were isolated from nosocomial infection. Materials and Methods: In this study, 51 isolates of Pseudomonas aeruginosa were collected from different wards of Imam Khomeini hospital of Ahvaz since October of 2014 until March of 2015. After identification test and antibigram, coding genes of antibiotic resistance and class I, II and III integrons were detected by polymerase chain reaction (PCR) method. Results: Tetracycline revealed the most resistance with 84% frequency in discreted isolates. In the encoding antibiotic resistance genes with a frequency of 94% was the most common blaTEM. Class I integron had 92% prevalence, class II Integron showed 52% prevalence and class III Integron demonstrated 17% prevalence. Conclusions: In Pseudomonas aeruginosa, class I integron was more prevalent than other integrons and the integrase gene was probably one of the causes of multiple antibiotic resistance in this bacteria. Moreover, frequency of integron III was reported 17%.
Authors and Affiliations
Parvin Hosseini Pour, Hassan Momtaz, Amir Arsalan Serajyan, Elahe Tajbakhsh
Keywords
Related Articles
- EP ID EP212140
- DOI -
- Views 155
- Downloads 0
The Cytotoxicity of Silver Nanoparticles Coated with Different Proteins on Balb/c Macrophage Cells
Background and Aims: Coated nanoparticles have different surface chemistry, aggregation, and interaction properties. The aim of this study was to investigate the cytotoxicity of silver nanoparticles AgNPs coated with dif...
The Effect of Vitamin E and Selenium on Sperm Chromatin Quality in Couples with Recurrent Miscarriage
Background and Aims: Due to the paucity of studies, association between the morphology and function of sperm and recurrent miscarriage (RM) is not yet completely known. Increased reactive oxygen species and decreased ant...
The Relation of Serum Bilirubin Level with the Severity and Complexity of Coronary Artery Disease and Long-term Outcomes in the Patients Undergoing Primary Percutaneous Coronary Intervention
Background and Aims: Bilirubin has been considered an antioxidant that protects against atherosclerosis. The aim of this study was to evaluate the relationship of serum bilirubin level with the severity and complexity of...
Advances in Hematopoietic Stem Cell Mobilization and Peripheral Blood Stem Cell Transplantation
Hematopoietic stem/progenitor cells (HSPCs) which give rise to different blood cell types are present within the bone marrow microenvironment, especially in flat bones such as skull, vertebrae, pelvis and chest. Interact...
The Effect of Echinops Lasiolepis Extracts, Native Plant of Yazd Province, on Peripheral Blood Mononuclear Cell Proliferation and IFN-γ Secretion
Background and Aims: The present study was performed to investigate the effect of Echinops lasiolepis extract on peripheral blood mononuclear cells (PBMCs) proliferation and interferon (IFN)-&gamma secretion. Materials...