Investigating genotoxicity of Eleusine indica by micronuclei assay in albino rats
Journal Title: African Journal of Biological Sciences - Year 2019, Vol 1, Issue 1
Abstract
Genotoxicity of Eleusine indica (Nkim enang: Efik) was investigated in the Wister strain albino rat (Rattus novergicus). Nine (9) male and nine (9) female rats were randomly assigned to three (3) groups, of which two were exposed to the aqueous extract of E.indica – Group A (control-no extract)), Group B (50 mg/kg BW of E. indica) and Group C (100 mg/kg BW of E. indica). This was administered to the rats by oral gavage for 14 days after which the peripheral blood from the tail tips were collected and assayed for the presence of micronuclei, following standard procedures. Proximate analysis and phytochemical screening of the herb extract was carried out. Results obtained showed that E. indica did not cause any significant (P > 0.05) increase in the incidence of micronucleated polychromatic erythrocytes in rat peripheral blood at any of the doses administered. The polychromatic: normochromatic erythrocyte (PCE: NCE) ratio was found to be in the range of 0.50 ± 0.11 to 0.55 ± 0.02. Also, the aqueous herb extract is rich in Carbohydrates (76.17%) and Tannins (21.76%). Mean body weights (MBW) of rats showed normal distribution throughout the duration of the investigation. The results of this study demonstrate that E. indica does not confer any genotoxicity in mammals. Further in-depth study on its efficacy is recommended.
Authors and Affiliations
Hannah Edim Etta, Ekei Victor Ikpeme, Effiom, Helen Offiong
Keywords
Related Articles
- EP ID EP469238
- DOI 10.33472/AFJBS.1.1.2019.33-40
- Views 223
- Downloads 0
Susceptibility of common weeds and cultivated crops in major maize growing agroecological zones of Uganda to viruses causing maize lethal necrosis disease
Maize lethal necrosis (MLN) disease is caused when maize plants become coinfected with Maize chlorotic mottle virus (MCMV) and potyviruses notably Sugarcane Mosaic Virus (SCMV). Apart from maize, little is known about su...
Detection of Helicobacter pylori among gastritis patients attending Nemba district hospital
Background: Helicobacter pylori (H. pylori) is a small, spiral-shaped bacterium that lives in the surface of the stomach and duodenum. More than half of the world’s population is colonized with H. pylori in the gastric m...
Population structure, fecundity and morphological characteristics of M. vollenhovenii (Herklots, 1857) on lower volta river basin channel, Ghana
Population structure, fecundity and morphological characteristics of M. vollenhovenii were studied around Lower Volta River, Ghana subject to dirt of information on this prawn species around the study location. The most...
Determination prevalence of cymothoid parasite fish families in Iwofe (Port Harcourt) Rivers state
The aim of this study is to provide information on the morphology of the Cymothoid parasites of Haemulidae and Elopidae from Iwofe in Port Harcourt determine physico chemical parameter of study area. To determine the per...
Investigating genotoxicity of Eleusine indica by micronuclei assay in albino rats
Genotoxicity of Eleusine indica (Nkim enang: Efik) was investigated in the Wister strain albino rat (Rattus novergicus). Nine (9) male and nine (9) female rats were randomly assigned to three (3) groups, of which two wer...