Investigating the Behavior of UVC Rays on the Inactivation of Staphylococcus Aureus from Simulated Contaminated Air Using a Probit Model
Journal Title: Advances in Clinical Toxicology - Year 2024, Vol 9, Issue 2
Abstract
Today, one of the most significant health challenges is controlling airborne microbial agents. Utilizing ultraviolet (UV) rays is a method to eliminate these microbial agents and can be an effective approach for disinfecting hospitals. The main mechanism in which UV radiation works is not by directly eliminating bioaerosols, rather by preventing their proliferation through damaging the outer structure of their cells. As a result, bioaerosols cease to proliferate and eventually die due to cell death. In this study, our objective is to determine the optimal duration of UV irradiation and temperature and pH required to achieve efficient removal of S. aureus bacteria. A suspension of bacteria was prepared using the 0.5 McFarland standard. The desired contaminated air was produced and blown into the reactor using an air pump. Sampling was done using a membrane biosterile filter with a pore diameter of 0.22 micrometers in various UV irradiation time, temperature, and pH. The number of colonies grown from the samples was counted. The removal efficiency of S. aureus bacteria was >70% after 10 minutes of irradiation, >80% after 20 minutes, and >95% after 30 minutes at a constant rate. Also, the actual bacterial removal efficiency differs slightly from the removal efficiency predicted by the probit model.
Authors and Affiliations
Leili M , Afrasiabi S , Khazaei M and Rostami R
Keywords
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- EP ID EP754555
- DOI 10.23880/act-16000303
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