Investigating the Prevalence of Mycobacterium Avium Subspecies Paratuberculosis (MAP) in Industrial Dairy herds using Ziehl-Neelsen Staining, Culture, and PCR in Mashhad, Iran
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2023, Vol 15, Issue 2
Abstract
Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease in wild and domestic ruminants. Clinically, infected cattle show emaciation symptoms, diarrhea, and death. Asymptomatic subclinical cases can intermittently shed MAP through feces and milk and infect other cattle animals, thus increasing the risk of infection. The purpose of the present study was to detect the prevalence of this disease in dairy cattle. For this purpose, 348 samples were randomly collected from 15 cattle farms. All fecal samples were subjected to ZN staining and PCR of nucleotide sequences related to specific MAP gene fragments (IS900, F57) and were cultured in Harold's egg yolk culture medium after being disinfected with solution (0.0% HPC75). PCR testing of 116 fecal samples (33.3%, CI:95% 28.3-38.2), ZN staining of 23 samples (6.6%, CI: 95% 4-9.2), and fecal sample culture of only 15 samples (4.3%, CI:95% 2.3-6.3,) were infected with MAP. Comparison of test results showed poor agreement (kappa statistic: 0.19) between ZN staining and PCR results and poor agreement (kappa statistic: 0.13) between PCR test and fecal culture. This study highlights the advantages of PCR for detecting MAP in subclinically infected cattle compared to ZN staining and fecal culture. Therefore, it can be used for early detection and control of MAP in cattle and at-risk populations.
Authors and Affiliations
Tahereh Gholamhosseini Moghaddam,Masoud Haghkhah,Gholam Reza Mohammadi,
Keywords
Related Articles
- EP ID EP722286
- DOI -
- Views 59
- Downloads 1
Effects of a sustained-release multi-trace element ruminal bolus on sex ratio, reproductive traits and lambs growth in synchronized Afshari ewes
The objective of the present study was to investigate the effects of a sustained-release multi-trace element ruminal bolus on sex ratio, the reproductive performance and lambs growth of Afshari ewes. Eighty Afshari cycli...
Effects of curcumin and its nano-micelle formulation on body weight, insulin resistance, adiponectin, and blood biochemical parameters of streptozotocin-induced diabetic rats
The effects of curcumin and its nano-micelle form on body weight, insulin resistance, adiponectin, and blood biochemical parameters of streptozotocin-induced diabetic rats were studied. Diabetes was induced in fifty male...
Serological study of BVDV and BHV-1 infections in industrial dairy herds of Arak, Iran
BVDV and BHV-1 are considered as worldwide, complicated and economically significant infections associated with a range of clinical syndromes in cattle. As clinical syndromes such as pneumonia, diarrhea, abortion and rep...
Lawsonia inermis possesses a significant analgesic activity compared to Waltheria indica, Moringa oleifera, Nigella sativa, and diclofenac in female Wistar rats
Pain is a severe symptom of many diseases, with an increasing percentage of people manifesting various types of pain. Medicinal plants provide analgesic potential with little toxicity. We performed this experiment to com...
Chronic endometritis-causing bacteria in Arabic mares
Bacterial infections of the uterus are known to be an important cause of infertility in the mare. The objective of this study was to determine the species of bacteria isolated from the uterus of infertile Arabic mares, a...