Investigation in the intergenic transcribed sequences to determination of genetic variation for Iranian species of salvia
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2017, Vol 11, Issue 6
Abstract
The nuclear ribosomal DNA internal transcribed spacer (ITS) has been established as a suitable genomic region to clarify genomic and phylogenetic relationships of plant species. This study aimed to evaluate the performance of ITS2 DNA barcoding for distinguishing Salvia species and determine genetic relationships among Salvia species from different regions of Iran. The seeds of 12 Salvia species were provided and planted. Total genomic DNA was extracted and ITS2 regions were amplified by PCR with specific primers and were sequenced by Bioneer Company. Genetic distance between Salvia species was estimated according to the Kimura 2-Parameter (K2P) model. ITS2 secondary structures predicted based on ITS2 databases. Multiple sequences were aligned using ClustalW algorithm and phylogenetic analyses were conducted using maximum likelihood (ML). The lengths of ITS2 sequences ranged from 270 to 282 bp and the mean of GC content was 59.3%. The greatest variation in helix length (10 to 22 bp) in the secondary structure of ITS2 at different species of Salvia was related to the third helix. All of the Salvia species formed five major clades. The results showed that the species of Salvia genus in Iran can be clearly distinguished from each other using ITS2 DNA barcoding.
Authors and Affiliations
Sedigheh Fabriki-Ourang
Keywords
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