INVESTIGATION OF IN VITRO METABOLITES OF ETODOLAC IN HUMAN HEPATIC CELL LINE AND CHICKEN LIVER TISSUE USING LC-MS/MS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 7
Abstract
Objective: The main objective of this study was to investigate the in vitro metabolic profile of etodolac (ETD) using normal human hepatic cell lines and chicken liver tissue, and to characterize the metabolites obtained using Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS).Methods: In the present study, the metabolic profile of ETD, a well-known non-steroidal anti-inflammatory drug (NSAID), was investigated in normal human hepatic cell lines and chicken liver tissue employing LC-MS/MS technique. The structural details on ETD metabolites were acquired using triple quadrupole mass spectrometer (LCMS-8040, Shimadzu). The metabolites were produced by incubation of ETD with the human hepatic cell lines and chicken liver tissue, at 37 °C for 24 h. The incubated extracts were analyzed with LC-MS/MS and their production spectra were acquired, interpreted and tentative structures were proposed.Results: Six phase I and phase II metabolites were successfully detected in the proposed study. The metabolic changes observed included-oxidation, N-acetylation, hydrogenation, decarboxylation, methylation and glucuronidation of dehydrogenated ETD. The tentative structures of the metabolites were postulated based on the chemical reactions predicted and the LC-MS/MS data obtained.Conclusion: The in vitro metabolites of ETD were successfully investigated and characterized in human hepatic cell lines and chicken liver tissue. Also, both the models were found to be equally effective for carrying out the in vitro metabolic study of ETD.Keywords: Etodolac, Metabolites, Hepatic cell lines, Chicken liver tissue, LC, LC-MS/MS
Authors and Affiliations
Divya Saxena, Shailesh Damale, Ajit Datar
Keywords
Related Articles
- EP ID EP576274
- DOI -
- Views 95
- Downloads 0
SYNTHESIS AND BIOLOGICAL EVALUATION OF SOME NOVEL SCHIFF BASES OF 2-QUINOLONES
A series of novel substituted 3-acetyl-1-(benzylideneamino) quinolin-2(1H)-one (1-12) have been synthesized by condensing different substituted 3-acetyl-1-amino-quinolin-2-one and aromatic aldehydes in alcohol medium. 3-...
ANTI-MRSA OF THE ETHYL ACETATE CRUDE EXTRACT FROM LASIODIPLODIA PSEUDOTHEOBROMAE IBRL OS-64, AN ENDOPHYTIC FUNGUS ISOLATED FROM LEAF OF OCIMUM SANCTUM LINN
Objective: To investigate the effects of ethyl acetate crude extract of an endophytic fungus, L. pseudotheobromae IBRL OS-64 which was isolated from leaf of Ocimum sanctum against the growth of methicillin-resistant Stap...
RAPID ISOLATION OF THE BIOACTIVE METABOLITE OF CYMBOPOGON PROXIMUS AND DEVELOPMENT OF LC METHOD
Objective: Development of a rapid isolation method to afford the bioactive metabolite (proximadiol: prox) from its herb (Cymbopogan proximus Stapf.) in the purest form and validate an LC method for its determination was...
QUALITY OF LIFE AMONGST BENZODIAZEPINE USERS PRE AND POST DEPRESCRIBING
Objective: The study aimed to analyze the Quality of Life (QoL) of patients utilizing benzodiazepines (BZDs). Methods: A prospective interventional study was conducted among 109 patients who met the inclusion criteria....
EFFECT OF MOMORDICA CHARANTIA FRUITS ON STREPTOZOTOCIN-INDUCED DIABETES MELLITUS AND ITS ASSOCIATED COMPLICATIONS
Objective: The present study was undertaken to assess the effect of Momordica charantia fruits on diabetes mellitus and its related complications.Methods: Crude powder, aqueous, 95% and 50% ethanol extract of Momordica c...