Investigation of P16 gene hypermethylation in serum of gastric cancer patients in Ardabil province
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 7
Abstract
Gastric cancer is the second leading cause of cancer death in the world. There is evidence that shows genetic and epigenetic change in stomach cancer is as a result of a variety of oncogenes, tumor suppressor genes, genes that repair the DNA, genes regulating cell cycle and cell adhesion molecules. P16 is an Inhibitor of protein kinase that is dependent on Cyclin D. Inactivation of this gene as a tumor suppressor gene, cause to cancer. This study was conducted to Investigation the P16 hyper-metylation status in serum of gastric cancer patients in Ardabil province as the prognostic factor for early diagnosis of gastric cancer. 82 Blood samples of patients with gastric cancer was prepared from Aras clinic of Imam Khomeini hospital. After DNA extraction from serum, using methylation-specific PCR assay it was examined the existence of hypermethylation in the P16 gene promoter. Incidence of free DNA in the serum of patients was 59.8%. P16 gene promoter was hyper methylated in 20.4% of subjects. Data analysis showed a statistically significant association between P16 methylation status and types of the adenocarcinoma. But there was no statistically significant association between methylation status of P16 with other features (age, sex, tumor location). This study showed P16 gene methylation of occur in some of gastric cancer patients. This result suggests that p16 methylation may be a prognostic marker for early diagnosis of gastric cancer.
Authors and Affiliations
Ebrahim Vosoughi| Azerbaijan National Academy of Science, Genetic Resources Institute, Baku, Azerbaijan, Department of Genetics, Medical Genetics lab, Imam Hospital, Ardabil University of Medical Sciences, Ardabil, I. R. Iran, Mazandaran University of Medical Science, Ramsar International Branch, Ramsar, I.R. Iran, Roya Osoli| Department of Genetics, Medical Genetics lab, Imam Hospital, Ardabil University of Medical Sciences, Ardabil, I. R. Iran, Zahra Moosavi| Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil,I.R. Iran, Mohammad Mazani| Department of Genetics, Medical Genetics lab, Imam Hospital, Ardabil University of Medical Sciences, Ardabil, I. R. Iran, Homa Akhavan| Faculty of Medicine, Uromia University of Medical Science, Uromia,I.R.Iran, S. Farimah Elyasi| Faculty of Medicine, Uromia University of Medical Science, Uromia,I.R.Iran, Soha Sadough| Mazandaran University of Medical Science, Ramsar International Branch, Ramsar, I.R. Iran, Tahira Asgarova| Department of Medical Genetics and Biochemistry Azerbaijan Medical University, Baku, Azerbaijan, S. Saied Hosseini-Asl| Department of Genetics, Medical Genetics lab, Imam Hospital, Ardabil University of Medical Sciences, Ardabil, I. R. Iran, Correspondence e-mail: saied.hosseiniasl@arums.ac.ir
Keywords
Related Articles
- EP ID EP11787
- DOI -
- Views 280
- Downloads 9
HEMOGLOBIN A1C INDUCED DOWN-REGULATION OF CD36 OF PLASMODIUM FALCIPARUM PARASITIZED RED CELL
Objective: High values of glycosylated hemoglobin have been found to correlate with decreased deformability of erythrocyte. CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of ma...
SCOLIOSIS CORRECTION IN CHILDREN-ANAESTHETIC CHALLENGE: A CASE REPORT
Kyphoscoliosis is a challenging surgery to surgeons but even more challenging to anaesthesiologist to give anaesthesia and maintain it throughout the surgery and post operative pain relief and ventilation. Here we are...
Detection of mecA Gene Associated with Methicillin Resistant Staphylococcus aureus and its Alternatives using Nanoparticles and Chia Seeds
Staphylococcus aureus, a member of the family Micrococcaceae, is a Gram-positive coccus whose cells tend to occur either singly or if dividing cells do not separate, form pairs, tetrads and distinctive irregular “grap...
Analysis of Hepatitis B Transmission Risk Factors in HIV Patients
Co-infection with Hepatitis B (HBV) virus and HIV is common due to similarity of their transmission methods. However, the prevalence of concurrent infection in different societies, shows the crucial role of various risk...
Investigating the Status of Respect Patient's Safety from Nurse’s View in Imam Sajad Educational and Therapy Hospital, Yasuj in 2015
Health care organizations in Iran, should always strive to improve patient safety and quality of their health care. The aim of this study was to assess the status of respect patient's safety from nurse’s view in Imam...