Investigation of Programmed Cell Death-1 (PD-1) Gene Variations at Positions PD1.3 and PD1.5 in Iranian Patients with Non-small Cell Lung Cancer
Journal Title: Middle East Journal of Cancer - Year 2018, Vol 9, Issue 1
Abstract
Background: Tumor cells express PD-1 ligands to bind PD-1 on immune cells and escape immune responses. In the present study, we aimed to investigate whether single nucleotide polymorphisms at positions PD1.3 (+7146, rs11568821) G/A, and PD1.5 (+7785 C/T, rs2227981) may be considered risk factors for susceptibility to nonsmall cell lung cancer in the Iranian population. Methods: This study enrolled 206 histopathologically confirmed lung cancer patients and 173 age/sex matched healthy controls. We performed PCR-RFLP to determine the genotypes of the extracted genomic DNA. Results: The frequencies of PD1.3 GG, GA and AA genotypes were 171 (83%), 31 (15%) and 4 (1.9%) out of 206 patients, and 144 (83.2%), 26 (15%), and 3 (1.7%) out of 173 controls, respectively. The frequencies of PD1.5 CC, CT and TT genotypes were 78 (37.9%), 100 (48.5%), and 28 (13.6%) in patients, and 60 (34.7%), 89 (51.4%), and 24 (13.9%) in controls. There were no significant differences in genotype analysis between patients and controls at positions PD1.3 (P=0.98) or PD1.5 (P=0.80). No significant differences existed in the frequencies of alleles and haplotypes between the two groups (P>0.05). Conclusion: Our data have indicated no association between PD1.3 (+7146) G/A and PD1.5 (+7785) C/T with susceptibility to non-small cell lung cancer. Investigation of other PD1 genetic variations and emerged haplotypes are required to completely define the role of PD1 genetic variations in susceptibility to lung cancer.
Authors and Affiliations
Zahra Pirdelkhosh, Tohid Kazemi, Mohammad Reza Haghshenas, Mohammad Ali Ghayumi, Nasrollah Erfani
Keywords
Related Articles
- EP ID EP302516
- DOI -
- Views 95
- Downloads 0
Epidemiology of Lung Cancer in Iran: Sex Difference and Geographical Distribution
Background: Lung cancer is one of the most common cancers and most frequent cause of cancer-related death worldwide. In Iran, this cancer ranks second in cancerrelated deaths for men and third for women. The aim of this...
A Model to Predict Breast Cancer Survivability Using Logistic Regression
Background: Breast cancer is the most common type of cancer amongst women worldwide. Considering its high incidence, effective detection and prognosis of this type of cancer may have a significant effect on reducing expe...
Evaluation of the Cytotoxic Effects of Ciprofloxacin on Human Glioblastoma A-172 Cell Line
Background: Glioblastoma multiforme, the most common, aggressive malignant brain tumor which affects patients of all ages, is principally resistant to treatment. Ciprofloxacin is an antibiotic that belongs to the fluoroq...
A Comparison of Endothelial Cell-Selective Adhesion Molecule and von Willebrand Factor Expression in Breast Cancer Growth and Metastasis
Background: Angiogenesis is the process of new blood vessels formation that contribute to tumor growth and metastasis. Endothelial cell-selective adhesion molecule is one of the proteins that expresses in vascular endoth...
Radiation Organ Dose Measurement and Cancer Risk Estimation in CT Examination on Trauma Patients
Background: This study intended to measure radiation doses to various organs and calculate the risk of cancer incidence from neck computed tomography and head computed tomography scans of trauma patients by using a therm...