Investigation of Protamine Genes (PRM1, PRM2) Polymorphism and Its Impact on Sperm Toroid Integrity in Males from Ahmedabad (India)
Journal Title: Asian Journal of Medicine and Health - Year 2017, Vol 7, Issue 1
Abstract
Aim: Although infertility rates have been escalating in the past few years, there are very few studies directed towards its investigation, especially for males in the region of Ahmedabad, India. Hence the present study was aimed at identifying polymorphisms in the protamine genes (PRM1, PRM2) and their possible impact on sperm chromatin, in order to determine molecular changes which could impede sperm fertilizing ability. Methodology: Infertile patients (Group II; n=23) with history of failure of in-vivo and in-vitro fertilization were included in the study. Normal, fertile age-matched men were selected as controls (Group I; n=25). Sperm Toroid Integrity (STI) assays was used to analyze Protamine-DNA binding efficiency. Sequencing was carried out for the protamine genes "PRM1 and PRM2" to screen for relevant single nucleotide polymorphisms (SNPs) that could alter the protein structure and its function of compact DNA binding, possibly impairing sperm fertilizing potential. Results: A significantly higher percentage of protamine-DNA dissociation and partial toroid disruption was observed in the infertile cases. However, sequencing yielded only a single distinct SNP at nucleotide 239 (rs737008) in PRM1 gene. Conclusion: The study revealed therefore that although the sequence of PRM genes and the resultant protamine proteins may not be altered, a loose protamine-DNA association and a disturbed toroid assemblage could render the DNA vulnerable to external pressures, leading to poor sperm function.
Authors and Affiliations
Highland Hyacinth, Rajput Nidhi, Sharma Rishika, Patel Kamini
Keywords
Related Articles
- EP ID EP336711
- DOI 10.9734/AJMAH/2017/35811
- Views 94
- Downloads 0
Surgery as a Career Choice among Rotatory Interns in North Central Nigeria – A Multi-center Study
Background: The future specialty of choice of interns determine the composition of medical practitioners workforce and the factors that underline this choice needs to be explored to help in healthcare planning and polic...
Low Prevalence of Helminth Infections among Primary School Children in the Volta Region of Ghana
Soil transmitted helminths (STH), as well as Schistosoma haematobium and mansoni, are very common helminth infections among children in Sub-Saharan Africa (SSA). This study aimed at evaluating the prevalence of helminth...
An Audit on Intravenous Drug Preparation and Administration in Various Departments of a Tertiary Care Hospital
Background: Intravenous administrations have a high incidence of errors but there is limited evidence of associated factors or error severity. Objective: To evaluate and categorise the attentiveness and negligence of nur...
Co-inheritance of Glucose-6-phosphate dehydrogenase Deficiency and Sickle Cell Traits in Sokoto Metropolis
Background: Transfusion from glucose-6-phosphate dehydrogenase (G-6-PD) deficient and Sickle cell traits (HbAS) blood constitute a major health burden and social challenge, especially to jaundiced neonate and sickle cell...
Head and Neck Sarcomas: Demographic and Histological Characteristics in a Tertiary Hospital of a Developing Country
Background: Head and neck sarcomas are rare connective tissue malignancies affecting all age groups and any of the anatomical sites of the head and neck region. Aim: Is to describe the demographic, anatomic and histologi...