Investigation of the acute effect of leptin on the inhibition of glycogen catabolism by insulin in rat liver perfused in situ.
Journal Title: Pharmacological Reports - Year 2009, Vol 61, Issue 2
Abstract
Leptin, a cytokine secreted by adipose tissue, has been implicated in the insulin resistance associated with obesity. Here we examined the acute influence of leptin at physiological (10 ng/ml) and supraphysiological (50 ng/ml and 100 ng/ml) concentrations on the inhibition of glycogen catabolism promoted by insulin in rat liver perfusion experiments. Perfusion of the liver with insulin (20 microU/ml) decreased the activation of glucose production (p < 0.05) and glycogenolysis by cAMP (3 microM). However, the infusion of leptin, at concentrations similar to those found in non-obese (10 ng/ml), obese (50 ng/ml), and morbidly obese (100 ng/ml) individuals did not influence the acute inhibitory effect of insulin (20 microU/ml) on glucose production and glycogenolysis stimulated by cAMP (p > 0.05).We conclude that neither physiological nor supraphysiological concentrations of leptin directly influence the inhibition of glycogen catabolism promoted by insulin in rat liver perfused in situ.
Authors and Affiliations
Erica Mario, Eledir Leonardo, Bruna Bassoli, Priscila Cassolla, Glaucia Borba-Murad, Roberto Bazotte, Helenir de Souza
Keywords
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