INVESTIGATION OF THE ANTIFUNGAL EFFECT OF THYMBRA SPICATA L. AND ROSMARINUS OFFICINALIS L. ESSENTIAL OILS ON FUSARIUM OXYSPORUM F.SP. CUCUMERINUM AND FUSARIUM OXYSPORUM F.SP. MELONIS
Journal Title: Black Sea Journal of Agriculture - Year 2018, Vol 1, Issue 2
Abstract
This study was carried out to determine the antifungal activity of Thymbra spicata L. and Rosmarinus officinalis L. plant essential oils. In this context, the antifungal effect of 0 (Control), 0.5, 1, 2, 4, 8 µlpetri-1 doses of plant essential oils on Fusarium oxysporum f. sp. cucumerinum (FOC) and Fusarium oxysporum f. sp. melonis (FOM) was identified. In order to determine the antifungal effect of essential oils, the filter paper adhered to the covers of the petri dishes was impregnated with the micropipette. Additionally, it was suggested that the plant essential oils inhibited the mycelial growth of FOM and FOC compared to the control. As a result of the study, 8 µlpetri-1 dose of T. spicata essential oil inhibited the mycelium growth of FOC and FOM by 100%. On the other hand, it was determined that 8 µlpetri-1 dose of R. officinalis essential oil inhibited the mycelium growth of FOC and FOM by 61.32% and 71.97%, respectively. In the study, LC50 and LC90 values were also calculated by carrying out a dose-effect study. The LC50 and LC90 values of FOM and FOC for T. spicata’nın essential oil were calculated as 0.58-1.26 µL and 0.46-1.07 µL, respectively, while the LC50 and LC90 values, of FOM and FOC for R. officinalis essential oil were calculated as 4.04-37.56 µL and 3.92-95.84 µL respectively. The T spicata, R. officinalis essential oils used in the experiment have a potential to be an alternative to fungus in controlling FOC and FOM diseases. It was concluded that the essential oils used in the experiment have a potential to be an alternative to synthetic pesticides in controlling diseases.
Authors and Affiliations
Yusuf BAYAN
Keywords
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