Investigation of the effect of trimethylamine-N-oxide on the proinflammatory cytokine genes expression in U937-derived macrophages
Journal Title: مجله علمي دانشگاه علوم پزشكي كردستان - Year 2018, Vol 23, Issue 95
Abstract
Background and Aim: Atherosclerosis is known as a multifactorial inflammatory disease. Trimethylamine N-oxide (TMAO) as a risk factor, has a potential to trigger or enhance the immune inflammatory reactions in atherosclerosis. Yet, The exact mechanism by which TMAO induces inflammation during atherosclerosis is not well understood. The present study was designed to evaluate the expression of IL-1β, IL-6, and TNF-α mRNA in response to treatment of macrophages with different concentrations of TMAO. Material and Methods: In this experimental in-vitro study, U937-derived macrophages were treated with different concentrations of TMAO (37.5, 75,150 and 300 µM) for 24 h. A group of cells were also treated with tunicamycin as positive control for stress. RT-qPCR was used to evaluate the expression of IL-1β, IL-6, and TNF-α mRNA levels. One-Way ANOVA and Post-hoc Dunnett test were used to compare the mean value of every group with that of control group. Results: Although TMAO increased expression of IL-1β, IL-6, and TNF-α mRNA, only 300 µM of TMAO significantly increased expression of IL-1β mRNA compared to the control cells (P<0.001). Tunicamycin increased expression of IL-6 significantly. Conclusion: The results of this study showed that among the above mentioned cytokines, IL-1β as a proinflammatory cytokine had a greater role in inflammatory reactions, induced by TMAO as a risk factor for atherosclerosis.
Authors and Affiliations
PhD M Abdi, PhD Z Vahabzadeh, A Ghanivash, PhD Candidate L Farhadi, PhD MS Hakhamaneshi, P Andalibi
Keywords
Related Articles
- EP ID EP364291
- DOI -
- Views 63
- Downloads 0
Effect of cardiac rehabilitation on physical and mental health components: a randomized controlled clinical trial
Background and Aim: Cardiovascular disease can affect physical and mental conditions. The purpose of the present study was to investigate the effect of phase 1 and 2 of cardiac rehabilitation on physical and mental healt...
Risk assessment of heavy metals in lipstick and hair dye cosmetics products in Sanandaj
Background and Aim: Human exposure to heavy metals causes health problems and use of cosmetic products; especially lipstick and hair dye contaminated by metals is considered a way of exposure to these agents. The aim of...
The effects of endurance training and whey protein supplementation on inflammation and insulin resistance in the rats fed with high-fat diet
Background and Aim: Whey protein has been known to be an excellent prophylactic agent against obesity. The aim of this study was to assess the effect of endurance training and whey protein supplementation on TNF-α levels...
Nurses’ mental health in Iran: A national survey in teaching hospitals
Background and Aim: Mental health disorders are common problems among health care professionals such as nurses, which can create great problems .The aim of this study was to evaluate mental health status in nursing perso...
Isolation and identification of Clostridium difficile from ready-to-eat vegetable salads in restaurants of Tabriz by Real-time PCR and determination of the antibiotic resistance pattern
Backgrounds and Aim: Clostridium difficile has been identified as a pathogen in antibiotic associated diarrhea, pseudo-membranous colitis. The ready-to-eat vegetable salads (REVS) are one of the possible sources for tra...