Investigation of the Prevalence of vanA and vanB genes in vancomycin resistant enterococcus (VRE) by Taq Man real time PCR Assay
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2013, Vol 3, Issue 4
Abstract
Objective: Enterococci are important nosocomial agents and due to their potential antimicrobial resistance they have a significant role in the dissemination of resistance genes. Currently, these species are described as healthcare concern. The aim of this study was to determine vanA and vanB genes in vanComycin resistant enterococci (VRE) strains isolated from the various clinical samples in the hospitals in Iran. Methods: Susceptibility of 235 strains to vanComycin was screened as minimum inhibitory concentration (MIC) by E-test. The genes encoding modifying vanComycin precursor’s dipeptide termini named as vanA and vanB genes were targeted by Taq Man real time PCR assay in vanComycin resistant and vanComycin intermediate resistant Enterococcus faecalis and Enterococcus faecium strains. Results: A total of 235 enterococci were isolated from the clinical specimens. One hundred and ninety three (82.1%) of them were defined as E. faecalis, 33 (14.0%) E. faecium, 1/235 (0.4%) E. avium, 1/235 (0.4%) E. raffinosus and 7/235 (3.0%) E. galinarium. The prevalence of vancomycin resistance was 13.6% (32/235) consisting of 18/235 (7.7%) E. faecalis and 6.0% (14/235) E. faecium. Among the 32 VRE strains, a total of 36 vanA and vanB genes were detected (some isolates had both vanA and vanB genes). These resistance genes were not detected in 5 out of 32 (15.6%) isolates. Conclusion: E. faecalis was more common in clinical samples and vanA (58.3%) gene was the predominant gene among the VRE isolates. The current study showed that Taq Man real time PCR assay is the useful, precise and rapid detection of vanComycin resistance genes. J Microbiol Infect Dis 2013;3(4): 192-198 Key words: VRE, Taq Man real time PCR, vanA, vanB, Enterococcus faecalis, Enterococcus faecium
Authors and Affiliations
Bahman Mirzaei, Taghi Farivar, Puran Juhari, Masume Mehr, Ryhane Babaei
Keywords
Related Articles
- EP ID EP157256
- DOI 10.5799/ahinjs.02.2013.04.0107
- Views 119
- Downloads 0
Uygun çevre koşullarında Streptomyces albolongus ile oluşan biyoaktif metabolit üretimi
Amaç: Günümüzde konvansiyonel antibiyotiklere karşı artan direnç riski nedeniyle yeni antibiyotiklere gereksinim vardır. Bu çalışmada yeni bir antimikrobiyal bileşiğin elde edilmesi denenmektedir. Yöntemler: Bir aktino...
Skin infection caused by Burkholderia thailandensis: Case report with review
Burkholderia thailandensis is genetically closed to Burkholderia pseudomallei, which causes melioidosis. The bacterium inhabits the environments of tropical regions including those in Southeast Asia and the Northern part...
Cerebellar involvement that occurred during treatment of Legionella pneumonia: A case report
Legionnaires’ disease can appear with different levels of severity. A case of a previously healthy lady with communityacquired pneumonia who progressed to severe acute respiratory distress syndrome and developed cerebel...
Kronik hepatit B hastalarında Entacavir tedavi sonuçları
Amaç: Bu çalışmada kronik hepatit B hastalarında 96 haftalık Entekavir (ETV) tedavi etkinliğinin ve güvenliğinin değerlendirilmesi amaçlandı. Yöntemler: Kronik Hepatit B tanısıyla 38 hasta çalışmaya alındı. Entekavir te...
Anti-nükleer antikor test sonuçlarının klinik uygulamada değerlendirilmesi
Amaç: Bu çalışmanın amacı 2009-2011 yıllarında laboratuvarımızda yapılan ANA (antinükleer antikor) tetkik sonuçlarının retrospektif olarak değerlendirilmesidir. Yöntemler: Bu dönemde laboratuvarımızda toplam 5068 serum...