Ion-pair HPLC Method for the Quantification of Metformin in Human Plasma and Its Application to a Pharmacokinetic Study
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 9, Issue 4
Abstract
Aims: To develop and validate a rapid, selective and sensitive ion-pairing HPLC-UV method for the determination of metformin in human plasma, using a conventional reverse phase column. Study Design: Experimental study. Place and Duration of Study: Department of Department of Biomedical Sciences, Faculty of Medicine, University of Medicine, “Mother Tereza” hospital center, between November 2014 and February 2015. Methodology: Ion-pair separation followed by UV detection performed on deproteinised and dichloromethane washed plasma samples was chosen for the determination of metformin. The separation was performed on an analytical LiChrocart® 100 RP 18 (125 × 4.0 mm i.d., 5 µm particle size) C18 column. A mobile phase consisting of acetonitrile and 10 mM sodium phosphate buffer (pH=6.0; 32.5:67.5, v/v) and sodium dodecyl sulfate (0.3%) was pumped at an isocratic flow rate of 1.25 mL/minute, and quantification was achieved at 236 nm using a UV/Vis DAD. Results: The calibration curves were linear (r > 0.9998) in the concentration ranges of 50-1600 ng/mL for metformin in plasma. The assay enables the measurement of metformin for therapeutic drug monitoring with a minimum detectable limit of 18 ng/ml. The coefficients of variation for inter-day and intra-day assay were within the range of clinical usefulness. Absolute recovery was found to be > 90% for all three concentrations of plasma quality controls studied. Conclusion: The proposed method was found to be rapid, precise and accurate for quantification of metformin in human plasma. This method was successfully applied to a pharmacokinetic study at humans through oral administration.
Authors and Affiliations
Eva Troja, Leonard Deda, Gëzim Boçari
Keywords
Related Articles
- EP ID EP342224
- DOI 10.9734/BJPR/2016/21436
- Views 108
- Downloads 0
Application of Alumina-coated Carbon Nanotubes in Removal of Tetracycline from Aqueous Solution
The alumina-coated multi walled carbon nanotubes (Al2O3-coated MWCNTs) was used for removal of Tetracycline (TC) from aqueous solution in batch systems. The adsorption of TC occurred by studying the effects of adsorbent...
Comparative Effect of Antidepressants (Duloxetine) and NSAID (Dexibuprofen) in a New Rat Model of Chronic Pain Induced Depression Associated with Monosodium Iodo Acetate (MIA) Induced Osteoarthritis in Rats
Objective: To evaluate the role of anti-depressants (Duloxetine) and NSAID (Dexibuprofen) in a new rat model of chronic induced depression. Methods: Twenty four male wistar rats were divided into 4 groups of 6 animals e...
Assessment of Entrepreneurial Traits and Intention of Pharmacy Students in Obafemi Awolowo University, Ile-Ife, Nigeria
Background: Sub-optimisation in utilisation of professional skills often poses major practice challenge to young pharmacists in institutional health care settings hence the need to redirect their focus to employment-prom...
Antiplasmodial Activity of the Root Extracts of Cochlospermum tinctorium in Mice Experimentally Infected with Clinical Isolates of Plasmodium berghei berghei (NK 65)
Aims: To determine in vivo the antiplasmodial activity of two chromatographic fractions obtained from Cochlospermum tinctorium A. Rich root bark extract against Plasmodium berghei berghei in mice. Methodology: Two chroma...
Anti-Diarrhoea Property of Crude Aqueous Leave Extract of Red Apple Psidium guajava in Castor Oil-Induced Diarrhoea in Rats
Objective: To investigate the anti-diarrhoea property of aqueous leaves extract of Psidium guajava (red apple guava leaves) in castor oil-induced diarrhoea in rats. Methods: Five groups of five rats each orally received...