ISOCRATIC SEPARATION OF FOUR BETA BLOCKERS WITH AMLODIPINE BY C18 RP-HPLC: APPLICATION TO AMLODIPINE DETERMINATION IN PHARMACEUTICAL DOSAGE FORMS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 6
Abstract
This method described for the successful separation of a beta blockers with amlodipine by RP-HPLC on a C18 column with UV detection. One of the key goals of High Performance Liquid Chromatography technique is to achieve a consistent and reproducible separation. A simple, precise, selective and sensitive HPLC method was developed and validated for determination of five anti-hypertensive agents, atenolol hydrochloride, metoprolol succinate, propranolol hydrochloride, amlodipine besylate and nebivolol hydrochloride with application to estimation of amlodipine besylate. RP-HPLC method was developed by using Welchrom C18 column (4.6 mm i.d. X 250mm, 5µm), Shimadzu LC-20AT ProminenceLiquid Chromatograph. The mobile phase composed of 10mM Phosphate buffer (pH3.0, adjusted with triethylamine): acetonitrile (50:50, v/v). The flow rate was set to 1.0ml/min with the responses measured at 235nm using Shimadzu SPD-20A Prominence UV-Visible detector. This method provides effective and reproducible separation of five anti-hypertensive agents less than 6 minutes. The retention times of atenolol hydrochloride, metoprolol succinate, propranolol hydrochloride, amlodipine besylate and nebivolol hydrochloride were found to be 2.310 min, 2.830 min, 3.473 min, 4.260 min and 4.960 min respectively. The statistical validation of the developed method was carried out according to ICH guidelines. Amlodipine besylate was found to give linear response in the concentration range of 2-10µg/ml. Recovery studies were performed to ascertain the accuracy by standard addition method and average recovery was found to be 99.83-100.40%. The LOD and LOQ were found to be 0.2251µg/ml and 0.6823µg/ml respectively. The developed method can be used for routine quality control analysis of amlodipine besylate in pharmaceutical tablet dosage form. It can also be extended for the determination of other above mentioned most commonly prescribed anti-hypertensive agents. The analysis yields a simple, rapid analysis, reduction in runtime, with excellent peak shape, high resolution as well as outstanding reproducibility.
Authors and Affiliations
Ravisankar Panchumarthy , Garikapati Rao
Keywords
Related Articles
- EP ID EP93370
- DOI 10.7897/2230-8407.04620
- Views 103
- Downloads 0
CARDIOTONIC ACTIVITY OF ISOLATED CARDIAC GLYCOSIDE FROM THE FRUITS OF CORCHORUS AESTUANS LINN
Preliminary phytochemical investigation of various extracts of fruits of Corchorus aestuans Linn showed the presence of cardiac glycosides, triterpenes, carbohydrates, saponins, flavonoids, tannins and fatty acids. An at...
SEMISOLID DOSAGE FORM OF PHENYTOIN SODIUM
Phenytoin sodium was formulated in a solid dosage form using different bases; o/w emulsion base, water soluble base, oleaginous base, and vanishing cream base. Bases were prepared in two different concentrations, 5% and...
PREPARATION AND CHARACTERIZATION OF FREEZE DRIED CRYSTALS OF IBUPROFEN
Ibuprofen, an anti-inflammatory drug, exhibits poor water solubility and flow properties.. Crystallization medium used for freeze dried crystals of Ibuprofen consisted of Isopropyl alcohol and water (50:50%) respectively...
AN INSIGHT TO OPHTHALMIC IN-SITU GEL: AN OVERVIEW
Ocular drug delivery has been a major challenging and interesting field for the pharmaceutical scientists due to unique anatomy and physiology of the eye. The major problem encountered in ocular drug delivery is the rapi...
CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL FROM CURCUMA AMADA ROXB.
This study was designed to examine the chemical composition and in vitro antioxidant activity of essential oil of Curcuma amada Roxb. The GC- MS analysis of the oil resulted in the identification of 12 compounds. β-myrce...