Isolasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Batang Nanas (Ananas comosus) Berdasarkan Variasi pH
Journal Title: Biogenesis: Jurnal Ilmiah Biologi - Year 2013, Vol 1, Issue 2
Abstract
This research aims to determine the highest protein content and the optimum activity of the enzyme bromelain extracted from pineapple stem. This research was conducted at the Laboratory of Microbiology Makassar Alauddin State Islamic University in May 2013. Variable in this study was the isolation and measurement of bromelain enzyme activity as the independent variable while the crude extract of pineapple stem by variations in pH as the dependent variable. The type of this research was experiments with bradford method for determination of protein content enzyme bromelain, with ammonium sulfate concentration variation for precipitation was 10-60%, as well as the determination of the enzyme bromelain activity at variation pH was 4.0; 5.0, 6.0; 7.0 and 8.0, at 650C temperature with incubation time was 10 minutes. Each stage has done three times in repetition and analyzed spectrometry. The results showed the highest levels of protein precipitation with ammonium sulfate at 60% was 37,785 mg/ml. While the optimum pH of the enzyme bromelain activity at pH 6.0 was 1.021 activity units /gram.
Authors and Affiliations
Nurhidayah Nurhidayah, Masriany Musa, Mashuri Masri
Keywords
Related Articles
- EP ID EP275455
- DOI 10.24252/bio.v1i2.457
- Views 323
- Downloads 0
Induksi Umbi Mikro Kentang Secara In Vitro Pada Suhu Tinggi Dengan Beberapa Tuber Promoter
Potato microtubers are the miniature of conventional seed tubers grown in the field. Producing potato microtuber in vitro at high temperature by applying many kinds of tuber promoting substances is similar to producing p...
Uji Ketahanan Tujuh Kultivar Jagung (Zea mays L.) Terhadap Penyakit Bulai (Peronosclerospora spp.)
Jagung (Zea mays L.) merupakan salah satu bahan makanan pokok masyarakat Indonesia. Bulai pada jagung adalah penyakit tanaman yang disebabkan oleh jamur Peronosclerospora spp. Jamur ini spesifik menyerang tanaman jagung,...
Aplikasi Teknologi Budidaya Melon (Cucumis melo L.) Kultivar Gama Melon Basket di Lahan Karst Pantai Porok Kabupaten Gunungkidul D.I.Yogyakarta
Melon (Cucumis melo L.) is one of favorite fruits for Indonesian. The fruit is sweet with good odor, and has a high nutrient content. Efforts to increase the production of melon can be reached by improving cultivation te...
Pemanfaatan Ampas Tahu Sebagai Pakan Ikan Lele (Clarias batrachus) Organik
This research was aimed to use tofu as organic feed for catfish and thus to create a product of organic fish feed made of tofu. Research was done in four steps, (I) Preparation of materials and equipment, (II) Production...
Aktivitas Antimikroba Hasil Fraksinasi Kortex Kayu Jawa (Lannea coromandelica (Houtt.) Merr.)
Kayu Jawa (Lannea cormendalica (Houtt.) Merr.) is native Indonesia plant, which became a typical plant for the treatment of wounds, dysentery, and other infectious diseases in some regions in Indonesia. An effort to expl...