Isolation and characterisation of Candida species from oropharyngeal secretions of HIV positive individuals
Journal Title: Nasza Dermatologia Online - Year 2011, Vol 2, Issue 3
Abstract
Material and methods: Oropharyngeal secretions were collected from 100 HIV positive individuals with CD4 counts less than 500, over a period of 4 months from June-September 2010. Samples were processed by standard methods for isolation of candida. Speciation was done by the color of growth on HiCrome agar, germ tube test, pellicle formation on SDA broth, chlamydospore production on CMA, growth at 450c and growth on Pal’s agar. Results: Out of 100, 63 samples were positive for fungal growth. Among 63, 17 samples yielded mixed growth and 46 samples yielded single isolates. Total isolates from 63 samples were 80. C. albicans was the predominant species both in mixed cultures (17 out of 34 mixed isolates i.e 50%) & single isolates (18out of 46 samples i.e 39%) and also in total isolates 35 out of 80 isolates, (43.7%) followed by C. tropicalis (17.6%, 20.9%, 18.7%), C. parapsilosis (20.5%, 6.9% and 12.5%), C. dubliniensis (11.8, 8.7%, 10%) in mixed, single and total isolates respectively. C. krusei and C. glabrata were obtained as single isolates. More positive cases were seen in individuals with CD4 count less than 200/cumm. Cultures (17 out of 34 mixed isolates i.e 50%) and single isolates (18 out of 46 samples i.e 39%) and also in total isolates (43.7%) followed by C. tropicalis (17.6%, 20.9%, 18.7%), C. parapsilosis (20.5%, 6.9%, 12.5%), C. dubliniensis (11.8, 8.7%, 10%) in mixed, single and total isolates respectively. C. krusei and C. glabrata were isolated as single isolates. More positive cases were seen in individuals with CD4 count less than 200/cumm.
Authors and Affiliations
Anaparthy Usharani, Bharathi M. , Cautha Sandhya
Keywords
Related Articles
- EP ID EP134643
- DOI -
- Views 81
- Downloads 0
Intense pulsed light: A promising therapy in treatment of acne vulgaris
Background: Medical treatment of acne vulgaris includes a variety of topical and oral medications. Poor compliance, lack of durable remission, potential side effects are common drawbacks to these treatment. Therefore, th...
Angiolymphoid hyperplasia with eosinophilia – A report of three cases
Angiolymphoid hyperplasia with eosinophilia (ALHE) is an uncommon, reactive vaso-proliferative disease, presenting with painless, vascular nodules in the dermal and subcutaneous tissues, sually seen in the head and neck...
Dermoskopia czerniaka skóry głowy-badanie przypadku i przegląd piśmiennictwa
Dermoskopia to aktualnie, nieinwazyjna metoda wizualizacji różnych struktur morfologicznych w obrębie melanocytowych zmian skórnych. W praktyce klinicznej, dodatni wynik badania jest uznawany z wskaźnik podwyższon...
Ulceras herpéticas perianales en pacientes inmunodeprimidos. Presentación de dos casos [Perianal herpetic ulcers in immunosuppressed patients. Report of two cases]
Genital herpes is the most common cause of ulcers in sexually transmitted diseases (STDs). It is presented as a single or multiple, painful injury, accompanied by bilateral, also painful, lymphadenopathy. In immunocompro...
komentarz: Herpes zoster ophthalmicus: kliniczny profil u dorosłych poniżej 30-go roku życia