ISOLATION AND CHARACTERIZATION OF A FLAVONOID FROM ETHANOLIC EXTRACT OF ALTERNANTHERA SESSILIS LINN.
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2017, Vol 6, Issue 1
Abstract
This study was performed to isolate and characterize the flavonoid present in Alternanthera sessilis Linn. Direct soxhlet extraction process was adopted for extraction by using 95% ethanol and vacuum evaporator was used for drying the extract. The concentrated ethanolic fraction was subjected to thin layer chromatography and column chromatography for isolation. The isolated compound was identified as a flavonoid by confirming the standard flavonoid tests: viz. Shinoda’s test. The Rf value of isolated flavonoid was calculate and different physical tests were also performed to find out its physical characteristics. Characterization of isolated flavonoid was done by FTIR, 1H NMR and MASS. The IR spectrum indicated the presence of hydroxyl and carbonyl functions. 13C NMR signal indicated the presence of hydroxyl group and unsaturated keto function and four methoxy group at different position of flavones skeleton. The 1H-NMR further showed the presence of three hydroxyl, four methoxy groups and three methine groups. On the basis of chemical and spectral analysis the structure was elucidated as 3´, 3, 6, 7-tetramethoxy - 4´, 5, 8-trihydroxy flavones. Keywords: Flavonoids, TLC, Column Chromatography, FTIR, NMR, MASS.
Authors and Affiliations
Mrinmay Das
Keywords
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