Isolation and characterization of an extremely heavy metal tolerant Sinorhizobium meliloti, utilizable for reclamation of polluted soils
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 6, Issue 2
Abstract
Soil heavy metals (HMs) have deleterious effects on Sinorhizobia and their symbiotic relationship with Legumes. Consequently, isolating HMs resistant strains and using them as inoculums in polluted soils is crucially important. In the present study, the effects of cadmium, lead and zinc on viability and nitrogen fixing potential of several native Sinorhizobium meliloti strains isolated from HMs polluted soils of Zanjan province- Iran was assessed. In this regard, several S. meliloti stains were isolated from alfalfa root nodules and their nitrogen fixation efficiencies were evaluated and compared based on symbiosis effectiveness and alfalfa shoot dry weights. Selective media containing different amounts of cadmium, lead and zinc were utilized to evaluate the tolerance rates of the isolate. Subsequently, the most HMs tolerant strains with high nitrogen fixation capability were evaluated and selected in pot experiments. Five HMS tolerant S. meliloti strains were selected and inoculated in culture media containing five different concentrations of cadmium, lead and zinc. S41 was recognized as the most HMs tolerant isolate with a symbiotic effectiveness of 139%. PCR amplification and sequencing of 16S-23S rRNA Intergenic Spacer Region was employed for molecular identification of this isolate which could significantly decrease the need for exogenous nitrogen.
Authors and Affiliations
Seyyed Mahdi Hosseinian, Shiva Khaledzadeh, Sara Nosrati, Ahmad Golchin, Esmail Memar-Kochehbagh
Keywords
Related Articles
- EP ID EP38237
- DOI -
- Views 162
- Downloads 0
Comparative analysis of physicochemical parameters and Bioaccumulation between Musa species
Banana plant plays a significant role in the north-eastern population of India. The plant can be truly called a kalpvriksh since all parts of this plant have been used by the people in Assam since ancient time. The plan...
Effects of low temperatures on the anti-oxidants activity of root in sensitive and tolerant genotypes of Barley (Hordeum vulgare L.)
Cold stress causes a disturbance in micro-organelles, transport of lipids in the plasma membrane, and production of reactive oxygen species (ROS) in plants, as well as growth and development prohibition, which subsequent...
Reducing fear of crime through spatial configuration analysis for urban sustainability in mixed-use neighbourhood
Fear of crime is an important social problem which has the negative impact on sociability and quality of life. The isolated streets are defined as urban spaces where fear of crime is highly perceived. Feeling safe depend...
On the incidences of oedipodinae (Orthoptera: Acrididae) from nara desert Khairpur, Sindh, Pakistan.
A detailed survey for the collection of short horned grasshoppers were carried out during the year 2012-2013 from different localities of Nara desert i.e. Choondiko, Khehwari, Tajal, Sikandarabad and Jubo. A total of 32...
Comparative study of endo-parasites from fecal samples of sambar (Rusa unicolor) and goral (Naemorhedus goral) in captivity
This project was designed to study the prevalence of endo-parasites in Sambar (Rusa unicolor) and Goral (Naemorhedus goral) kept at Jallo Wildlife Park and Lahore Zoo, Punjab, Pakistan. Fresh faecal samples were collec...