Isolation and characterization of Biosurfactant Produced by Soil Isolates Pseudomonas species and Rhodococcus sp
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
Biosurfactants due to their biocompatibility, biodegradability, nontoxicity and versatility over synthetic surfactants are gaining much importance over synthetic surfactants. The present study involves isolation of bacterial strains from hydrocarbon polluted areas of Hubli city and Karwar port and identified. The isolates were cultured on Bushnell Haas medium. Biosurfactant production was carried out in nutrient medium supplemented with hydrocarbon to minimise contamination. Efficiency of biosurfactants against different hydrocarbons was checked by Oil spread technique, Emulsification index and Emulsification activity. In the present study, soil isolate Pseudomonas sp was efficient in oil displacement and emulsification activity as compared to other isolates. While another soil isolate Rhodococcus sp was efficient in Emulsification index .The percentage yield of crude biosurfactant was also done which showed Pseudomonas sp to be the highest producer of biosurfactant. It is evident from the results that biosurfactants can be used in place of synthetic surfactants due to their efficiency and eco friendly nature.
Authors and Affiliations
Geetanjali R K, Prajnya A M, Shruti A J, Sunita N, Bhushan B K Potadar R. , Kalebar V. U, Shinde H Parimala C. P,, Shetti A. A, S V Hiremath*
Keywords
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- EP ID EP390902
- DOI 10.9790/3008-1205011720
- Views 87
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