ISOLATION AND CHARACTERIZATION OF ENDOPHYTIC AND RHIZOPHERIC BACTERIA ASSOCIATED SWEET-POTATO PLANTS CULTIVATED ON SOILS OF THE MEKONG DELTA, VIETNAM
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2017, Vol 6, Issue 1
Abstract
Rhizobacteria and endophytic bacteria in the acid sulphate rhizosphere soils and stems/roots of sweet potatoesgrown on acid-sulphate soilsof the Mekong Delta, Vietnam were studied. A total of 96isolates were isolated on two media (Burk’s N-free and NBRIP) and 260 endophytic isolates (128 isolates from LGI medium and 132 isolates from Nfb medium) from 60 rhizosphere soil samples were taken in threeregions (Tu Giac Long Xuyen, Dong Thap Muoi, Trung Song Hau) and all of themhave ability of nitrogen fixation and phosphate solubilization. The sequences from selected rhizobacteria and endophytic bacterial isolates (12isolates) were analysed 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (8F and1492R) and sequenced. The results showed they had high degrees of similarity to those of the GenBank references strains (between 98% and 99%). From 12isolates, 3belonged to Bacilli while Beta-proteobacteria and Gamma-proteobacteria were3 and 6strains,respectively.From these results showed that there are sixstrains asPseudomonas taiwanensis LA1-3-3, Bacillus circulans KG2-3-2, Klebsiella sp. HG5-3-2, Enterobacter asburiae KG9-2-1-1, Burkholderia metallica HG7-2-1-2, Enterobacter cloacae AG1-1-1-1 revealed promising candidates with multiple beneficial characteristics and they have the potential application as inoculants adapted to poor soils (unfertile soils) and local crops because they are not only famous but also safestrains for sustainableagriculture, especially for growing sweet potatoeson acid sulphate soilsin the future.
Authors and Affiliations
Ly Ngoc Thanh Xuan
Keywords
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