ISOLATION AND CHARACTERIZATION OF MDR BACTERIA FROM IN VITRO CULTURE OF BACOPA MONNIERA AND SUPPLEMENTATION OF NATURAL EXTRACTS TO CONTROL BACTERIAL CONTAMINATION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 11
Abstract
Objective: The purpose of the present study was to isolate and characterize bacterial contamination from in vitro culture of Bacopa monniera callus culture.Methods: The two selected isolates (1 and 2) were identified by morphological, biochemical and molecular (16S rRNA gene sequencing) methods. Beside this antibiotic resistance was also determined.Results: The isolates were identified as closely related to Enterobacter cloacae (KU350623) (Isolate 1) and Myroides odoratimimus (KU382740) (Isolate 2). The isolate 1 and 2 were found to be resistant to streptomycin (25 mcg), dapsone (10 mcg), erythromycin (15 mcg), chloroamphenicol (25 mcg) and ampicillin (10 mcg). Supplementation of the natural extract was tested to control the contamination due to these multi drug resistant bacteria. Aqueous and alcoholic leaf extracts of Azadirachta indica was added to plant tissue culture media i.e. MS media in order to control contamination.Conclusion: The present studies suggest using natural extracts supplementation as a promising strategy to control the in vitro bacterial contamination in plant tissue culture.
Authors and Affiliations
Yash Sharma, Manish Bhardwaj, Anshita Nagar, Neeta Bhagat
Keywords
Related Articles
- EP ID EP575890
- DOI 10.22159/ijpps.2016v8i11.13593
- Views 39
- Downloads 0
QUALITY OF LIFE AMONGST BENZODIAZEPINE USERS PRE AND POST DEPRESCRIBING
Objective: The study aimed to analyze the Quality of Life (QoL) of patients utilizing benzodiazepines (BZDs). Methods: A prospective interventional study was conducted among 109 patients who met the inclusion criteria....
SUPPLEMENTATION OF CALCIUM AND SELENIUM AGAINST CADMIUM INDUCED BIOACCUMULATION IN SELECTED TISSUES OF FRESH WATER FISH, OREOCHROMIS MOSSAMBICUS
Objective: Cadmium (Cd) is one of the most hazardous heavy metals in aquatic environments and could threaten aquatic organisms including fish. The present study was carried out to know the protective effects of calcium (...
DEVELOPMENT STATUS IN THE MEADOW OF NANOSTRUCTURE MAGNETIC DRUG DELIVERY SYSTEM AND ITS PROMISING APPLICATIONS
This article discusses about magnetic nanoparticles, their physico-chemical properties various applications in medicinal sectors and technology advancements. Superparamagnetic, high magnetic susceptibility, non-toxicity...
STUDY ON CAUSE–EFFECT RELATIONS AND OPTIMIZATION OF EXEMESTANE-LOADED NANOSTRUCTURED LIPID CARRIERS
Objective: Exemestane is an anti-breast cancer drug, possesses low water solubility and low permeability. This work aimed at the cause-effect relations and optimization of exemestane-loaded nanostructured lipid carriers...
ANTI-ARTHRITIC AND ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC LEAF EXTRACT OF ORMOCARPUM SENNOIDES
Objective: The study focuses on evaluating the anti-inflammatory and anti-arthritic activity of ethanolic extract of Ormocarpum sennoides (Os), a herb belonging to the family fabaceae. Both in vitro and in vivo methods w...