ISOLATION AND CHARACTERIZATION OF SECONDARY METABOLITES FROM PITHECELLOBIUM DULCE BENTH FRUIT PEEL
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: The present study describes the isolation and characterization of valuable secondary metabolites from the fruit peel of Pithecellobium dulce. Methods: Solvent extraction of the plant material using methanol followed by column chromatographic separation of the extract with solvents (Petroleum ether, ethyl acetate and methanol) of increasing polarity using silica gel yields four compounds (1-4). Results: The structures of the compounds stigmasterol (1), β-sitosterol (2), quercetin (3) and pinitol (4) were established on the basis of FTIR, 1H and 13C NMR; and mass spectra and in comparison with their literature values. Conclusion: Two sterols namely stigmasterol (1) and β-sitosterol (2), along with a flavonoid quercetin (3) and a cyclitol pinitol (4) have been isolated from the methanol extract of fruit peel of Pithecellobium dulce for the first time. Â
Authors and Affiliations
Sukantha T. A. , Shubashini K. Sripathi
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