Isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from COVID-19 patients in Shanghai
Journal Title: Shanghai Journal of Preventive Medicine - Year 2022, Vol 34, Issue 12
Abstract
ObjectiveTo isolate and study the biological characteristics of severe acute respiratory syndrome coronavirus 2 ï¼SARS-CoV-2ï¼ from feces of coronavirus disease 2019 ï¼COVID-19ï¼ patients.MethodsVero E6 cells were used for virus isolation and the isolated strains were tested by nucleic acid testï¼ immunofluorescence testï¼ virulence test and whole genome sequencing. 50% tissue culture infective dose ï¼TCID50ï¼ was calculated after the cell cultures of each generation were collectedResultsEight fecal specimens were inoculated with Vero E6 cells after treatment and cultured for 48 h. One specimen showed obvious cytopathic effect on Vero E6 cells. One SARS-CoV-2 out of 8 fecal samples from COVID-19 patients were isolatedï¼ and separation rate was 12.5%. The TCID50 of P1ï¼ P2 and P3 were 104.0/0.2 mLï¼ 104.5/0.2 mL and 104.75/0.2 mLï¼ respectively. Only one of the 8 stool samples had SARS-CoV-2 virus replication and amplificationï¼ and the Ct value of the nucleic acid detection was about 10. The sequence of the isolation was more than 99.99% homologous with that of Wuhan-Hu-1ï¼GenBank MN908947ï¼.ConclusionThe SARS-CoV-2 strain is isolated from the fecal samples of COVID-19 cases and is confirmed by genomic sequencing and immunofluorescence testï¼ which indicates the presence of live virus in feces of COVID-19 cases.
Authors and Affiliations
ZHOU Yanqiu,CUI Xiaoxian,MOU Jiabin,FANG Fanghao,TENG Zheng,WU Huanyu,CHEN Min,ZHANG Xi,
Keywords
Related Articles
- EP ID EP742254
- DOI -
- Views 27
- Downloads 0
Analysis of menstrual conditions and influencing factors in 281 women infected with COVID-19
Objective To investigate the menstrual conditions of women infected with COVID-19 in Shanghai and analyze the influencing factors.Methods From December 2022 to March 2023， menstrual data from 281 women infected with COV...
Application and comparison of three risk assessment methods for occupational health risk assessment of dust in a sanitary ceramic product manufacturing enterprise
Objective Three methods were applied to conduct occupational health risk assessment for the working positions exposed to silicon dusts in a sanitary ceramic manufacturing factory， and the evaluation results were compare...
A family clustering of coronavirus disease 2019 caused by asymptomatic infection
<b>Objective:To analyze a family clustering of coronavirus disease 2019 （COVID-19） associated with the exposure to an asymptomatic case， and to provide evidences of developing strategies for COVID-19 prevention. <b>M...
Air quality monitoring and correlation analysis in public areas of three subway stations in Shanghai
ObjectiveTo understand the correlation between air quality and pollutants in three underground subway stations in Shanghai and their influencing factorsï¼ in order to provide a reference for improving the ambient air qu...
Trend and gender difference of mortality rate of senile dementiaï¼ based on the method of annual percent change
ObjectiveTo investigate the characteristics of gender difference and the trend of the mortality rate of senile dementia in registered population in Shanghai from year 2002 to 2018ï¼ and to provide the basis for formulat...