Isolation and identification of ampicillin degrading bacterial strain from soil sample
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2016, Vol 4, Issue 10
Abstract
The pharmaceutical compounds cause problems mainly in the terrestrial and aquatic environment. It is due to the accumulation of these compounds. The pharmaceutical compounds are converting into nontoxic compounds after biodegradation by microorganisms. Ampicillin is a semisynthetic antibiotic and belongs to β-lactam group of antibiotic. The ampicillin was degraded by bacterial isolates AMP6 and AMP8. The initial concentration was 50ppm of ampicillin for isolation of bacterial isolates. The isolates were identified as Ochrobacterum intermedium and Bacillus oceanisediminis respectively by using 16s RNA sequencing. The concentration ranging from 50 ppm to 500ppm of ampicillin was considered for its biodegradation. After 6 days of incubation at 400ppm the Ochrobacterum intermedium degraded 12.97±0.11% and the degradation by Bacillus oceanisediminis was noticed as 11.98±0.15 %.
Authors and Affiliations
Gayatri Devi Singh, Dr. K. C. Gupta, Dr. Archana Shrivastav
Keywords
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