Isolation and identification of bacterial pathogens on the surfaces of gate passes from Umaru Musa Yar’adua University main gate
Journal Title: International Journal of Medical and Health Research - Year 2018, Vol 4, Issue 7
Abstract
The bacterial contamination of shared objects in workplace settings could serve as a potential source for community acquired infections. The aim of this study was to isolate, identify and determine the bacterial load on the gate passes of Umar Musa Yar’adua University. A total of 60 samples of gate passes were swabbed and analysed for bacterial contamination. Samples collected were inoculated in nutrient broth and incubated at 37oC for 24 hours after which they were plated out on nutrient agar, MacConkey and Mannitol salt agar (MSA) for bacterial isolation and for the estimation of the bacterial load. The samples were inoculated into nutrient broth after which they were serially diluted and plated out on nutrient agar and incubated at 37oC for 24 hours. A Quebec colony counter was used for colony count. Out of the 60 samples collected, 30 samples were collected from car gate pass and 30 from motorcycle gate pass. The bacterial isolates and their percentage prevalence include Enterobacter aerogenes (34.7%), Pseudomonas aeruginosa (30.6%), Staphylococcus aureus (22%) and Escherichia coli (12.2%). The results also show that the motorcycle gate passes had higher contamination with four organisms isolated and three organisms were isolated from car gate passes. The contamination by microorganisms of gate passes and transmission of infection can be minimized by practising simple hand hygiene.
Authors and Affiliations
Sani Aliyu, Khadija Hassan Mashi, Mohammed Al-Kassim Hassan
Keywords
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