Isolation And Identification of Candidal Species From Oral Cavity
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 12
Abstract
Introduction: The fungi are a diverse group of saprophytic and parasitic eukaryotic organisms. The most important oral yeasts belong to the genus Candida. In recent years there has been an increased interest in infections caused by the opportunistic pathogen Candida. The growing importance of Candida is in part related to the emergence of HIV infection and the more widespread use of immunosuppressive chemotherapy. Identification of infecting strains of Candida is important because isolates of Candidal species differ widely, both in their ability to cause infection and also in their susceptibility to antifungal agents. Aim:The aim of the study is to identify and isolate the candidal species in dental patients by culturing them using Sabouraud’s Dextrose Agar and CHROMagar medium Materials And Methods: A cross sectional study was done for a period of four months in which a total of 40 subjects 10 of them presenting with clinical oral manifestations of candidiasis, 10 of them who were denture wearers, 10 of them with poor oral hygiene, 10 patients with reasonably good oral hygiene were selected for the study were included in the study after obtaining informed consent from the Outpatient Department of Oral Medicine and Radiology, Meenakshi Ammal Dental College And Hospital, Chennai, India. Results: In our study we were able to isolate six different candidal species from oral caviy of patients which comprised of Candida albicans and five nonalbicans species which included Candida tropicalis, Candida krusei, Candida dubliensis, Candida parapsilosis and Candida glabrata. We found a statistically significant p value of less than 0.05 in assessing the various objectives in different species as a whole, but when analysed individually there was statistically significant differences observed in only two species C.albicans and C.tropicalis. Conclusion:On the whole it was observed that as the CHROMagar medium gives a presumptive identification within 48 hours, preliminary antifungal treatment can be administered with confidence while the confirmed identification is being obtained. The availability of this type of media not only facilitates the provision of rapid patient care, but may also assist to control the rise in antifungal agent resistance by reducing the time taken for presumptive identification of the organism at species level to start the therapeutic regime
Authors and Affiliations
Dr. Saraswathi K. Gopal, Dr. Supriya Manoharan, Dr. Arathy. S . Lankupalli
Keywords
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- EP ID EP250769
- DOI 10.9790/0853-1612057380
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