Isolation and identification of Clostridium difficile from ready-to-eat vegetable salads in restaurants of Tabriz by Real-time PCR and determination of the antibiotic resistance pattern
Journal Title: مجله علمي دانشگاه علوم پزشكي كردستان - Year 2017, Vol 22, Issue 89
Abstract
Backgrounds and Aim: Clostridium difficile has been identified as a pathogen in antibiotic associated diarrhea, pseudo-membranous colitis. The ready-to-eat vegetable salads (REVS) are one of the possible sources for transmission of C. difficile to human. The aim of the present study was isolation and identification of Clostridium difficile in ready-to-eat vegetable salads in the restaurants of Tabriz by Real-time PCR and determination of its antibiotic resistance pattern. Materials and methods: This was a cross-sectional study. A total of 60 ready-to-eat vegetable salads samples were collected randomly from restaurants in different regions of Tabriz from February to June 2015. After preparation and DNA extraction, Clostridium difficile was identified by Real-time PCR method. Disc diffusion method was used to determine antimicrobial resistance of the isolates to eight different antibiotics. Using SPSS 19 software, chi-square was used for data analysis. p ≤0.05 was considered significant. Results: Among that 60 samples, 8 (13.33%) were contaminated with Clostridium difficile. There was no difference in Clostridium difficle prevalence in different regions (p= 0.296). Among eight antibiotics used in this study, nalidixic acid with 8 isolates (100%) and Clindamycin with 7 isolates (87.5%) had the highest resistance rate. We found no resistance to metronidazole and vancomycin. Conclusion: The results of this study showed that the ready-to-eat salad vegetables can be a way for transmission of Clostridium difficle to humans. Therefore it is necessary to take necessary measures to prevent transmission of the infection through ready to- eat vegetable salads
Authors and Affiliations
BS H Kochakkhani, PhD M. H Moosavy, PhD P Dehghan
Keywords
Related Articles
- EP ID EP282911
- DOI -
- Views 70
- Downloads 0
Investigation of the frequency of meniscal ligament injuries and other abnormal knee injuries in the patients referring to MRI center of Shahid Ghazi Clinic in Sanandaj
Background and Aim: Knee is the largest synovial joint in the body. Knee joints are supported on each side by collateral, meniscal, anterior cruciate ligaments (ACL) and posterior cruciate ligaments (PCL). About 50% of p...
Evaluation of astaxanthin effects on the survival and proliferation of human adipose derived stem cells
Background and Aim: In order to enhance in vivo stem cell viability and considering similar anti-inflammatory and anti-apoptotic effects of human adipose derived stem cells (hADSCs) and astaxanthin (AST) and their abilit...
Serum procalcitonin level as a biomarker for the differential diagnosis between infectious and non-infectious systemic inflammatory response syndrome: a prospective case–control study
Background and Aim: Considering the importance of sepsis and its complications in the mortality of the patients admitted to different parts of hospital and the importance of early diagnosis and timely and appropriate tre...
The relationship between sensorimotor function of hemiplegic lower limb and independency in daily living activities in hemiplegic patients
Background and Aim: The aim of the present study was to find out the relationship between the various components of sensorimotor function of hemiplegic lower limb and independency in daily living activities in stroke pat...
The effect of endurance activity on miR-499 and sox6 genes expression in fast and slow twitch skeletal muscles
Background and Aim: Endurance activity can affect function and also factors involved in skeletal muscle transcription. The aim of this study was to investigate the effects of an endurance activity program on the Sox6 gen...