Isolation and Identification of Potential Gossypol Degrading Fungal Strains from Cotton Growing Soil
Journal Title: Microbiology Research Journal International - Year 2017, Vol 21, Issue 1
Abstract
Aim: To isolate and identify gossypol degrading fungal strains from cotton growing soil of Maharashtra and Andhra Pradesh States of India. Study Design: Experimental study. Place and Duration of the Study: Chemical and Biochemical Processing Division, ICAR-Central Institute for Research on Cotton Technology, Matunga (E), Mumbai – 400019, India during January to December 2014. Methodology: The isolation of fungi from soil samples was done by enrichment culture technique using gossypol as a sole carbon and energy source. The fungal isolates were inoculated in cottonseed cake (CSK) and incubated for 48 h at 30°C. The isolates were screened for gossypol detoxification by estimating free and total gossypol level in the fermented CSK. The potential gossypol degrading isolates were identified by 18S rDNA analysis. Results: Eight isolates (F1 to F8) were identified as potential gossypol degrading strains among the fifty nine isolates obtained. The new fungal isolates from F1 to F8 were identified as Aspergillus terreus (KP 264957.1), Lichithemia ramosa (KP 264961.1), Alternaria alternata (KP 264960.1), Fusarium equiseti (KP 26459.1), Fusarium chlamydospora (KP 264958.1), Fusarium sp. (KP 209032.1), Fusarium solani (KP 264956.1) and Fusarium thapsinum (KP 264962.1) respectively, based on 18S rDNA analysis. The maximum free and total gossypol reduction (%), 65.2 and 59.8 respectively was observed in Fusarium thapsinum (KP 264962.1) treated CSK. The protein content was higher in fungal treated than untreated CSK. Conclusion: The present study identifies new fungal strains capable of degrading toxic compound, gossypol in cottonseed cake.
Authors and Affiliations
Vellaichamy Mageshwaran, Sharmila B. Majee, Kuppusamy Pandiyan
Keywords
Related Articles
- EP ID EP315971
- DOI 10.9734/MRJI/2017/35364
- Views 65
- Downloads 0
Isolation and Antibiogram of Staphylococcus aureus from Patients Admitted at Specialist Hospital Okitipupa, Ondo State, Nigeria
Aims: To isolate and determine the antimicrobial resistance and susceptibilities of Staphylococcus aureus from patients hospitalized at State Specialist Hospital, Okitipupa, Ondo State, Nigeria. Study Design: Prospectiv...
Seroepidemiology of Incidentally Detected Asymptomatic HBsAg Positive Subjects from Southern State of India – A One Year Study
Introduction: Hepatitis B virus infection is assuming a silent epidemic phase in India. Majority of the chronically infected are asymptomatic and unaware of their status. This pool of individuals called; incidentally det...
Anaerobic Microbial Activities of a Nigerian Onshore Oil Production Facility that Uses Underground Water with Zero Sulfate Concentration for Injection
Aim: To evaluate the anaerobic microbial activities and souring potential of a Nigerian onshore oil production facility that uses zero sulfate underground water for injection. Methodology: Key functional group activities...
Comparison of Automated System Vitek-2 with Conventional Methods, for Identification and Antibiotic Sensitivity in Gram Negative Organisms
Background: Rapid bacterial identification and susceptibility testing improves patient therapy and outcome and decreases emergence of resistance. There is a need to provide rapid, efficient and accurate system for identi...
Evaluating Efficacy of Biosurfactants from Bacterial Isolates in Conferring Protection against Rhizoctonia and Sclerotium Infection in Wheat and Peanut Plants
Aims: To isolate biosurfactant producers from natural habitat and to test the antimicrobial activity of the extracted biosurfactant against fungal plant pathogens. Study Design: The present study was designed to achieve...