Isolation and Identification of Potential Probiotics Bacteria from the Gut of Oreochromis niloticus and Clarias gariepinus in Uganda
Journal Title: Microbiology Research Journal International - Year 2016, Vol 17, Issue 5
Abstract
Bacterial infections remain a hindrance to aquaculture expansion globally. Increased fish mortality and poor performance resulting from ill health has forced farmers to resort to the use of antibiotics globally. However, prolonged use of these drugs in aquaculture is becoming restrained as pathogens develop resistance to drugs and unpredicted long term effect on public health. Alternative approaches to control disease are proposed of which probiotics have come forward. Therefore, the purpose of this study was to identify potential probiotic bacteria in the guts of fish from different sampling sites around Kampala. Fish were obtained from selected cages, ponds, tanks and hatcheries around Kampala, including different parts of Lake Victoria. The fish were gutted and the guts aseptically swabbed with subsequent culture on both general purpose and selective media. The identification of various isolates was based on gram staining and biochemical tests. Probiotic screening was done using the agar spot method. Results revealed complete growth across all samples. The total microbial load was highest in the samples from the lake (1204.8±12.7 × 105cfu/g). Out of the three probiotic genera isolated, only Lactobacillus spp (LB) and Lactococcus spp (LC) showed antibacterial activity against selected pathogenic bacteria. The activity of LB was significantly (p< 0.0001) higher against Streptococcus spp (17.0±0.2 mm) as compared to Proteus at 9±0.02 mm and Pseudomonas (7.5±0.2 mm). Lactobacillus spp did not show any antimicrobial activity against Staphylococcus aureus. For Lactococcus spp, probiotic activity was only detected against Proteus spp (5.5±0.2 mm). Although our study shows that Lactobacillus spp and Lactococcus spp possess probiotic activity against a number of pathogenic bacteria, characterization of these isolates is paramount before further manipulation.
Authors and Affiliations
Charles Drago Kato, Mark Benjamin Mugaanyi, Samuel Majalija, Andrew Tamale, Nathan Lubowa Musisi, Asuman Sengooba
Keywords
Related Articles
- EP ID EP350735
- DOI 10.9734/BMRJ/2016/29721
- Views 61
- Downloads 0
Purification, Characterization and Applications of Proteases Produced by Bacillus amyloliquefaciens 35s Isolated from Soil of the Nile Delta of Egypt
Aims: The current study aimed at purifying and determining general characteristics of proteases produced by Bacillus amyloliquefaciens 35s isolated from the soil of Nile Delta of Egypt and to study its applications in en...
Antibacterial Activity of Croton bonplandianum (Bail.) Against Some Bacterial Isolates from Infected Wounds
Aims: The antibacterial activity of medicinal plants against human pathogens has been evaluated by a number of studies. However, a few research works have been done on pus cell pathogens. So this research work was aimed...
Effect of Acid Adaptation of Listeria monocytogenes on Its Mild Thermal Inactivation in a Simulated Fruit Juice Supplemented with Carvacrol
Aims: The use of mild heat treatment in combination with carvacrol has been reported as a possible way to quickly inactivate L. monocytogenes in fruit juices. This study aimed at assessing the possible effect of a prior...
Hepatitis C Virus Genetic Diversity and Drug Mutational Analysis in Cameroon: 1992 to 2013
HCV infection is endemic in Cameroon. A Direct acting agent (DAAs), sofosbuvir that target the HCV non-structural 5B RNA-dependent RNA polymerase (NS5B RdRp) has recently been incorporated into the standard of care in Ca...
Lactobacilli Cultures against Ochratoxin A Producing Moulds Isolated from Cocoa in the South West Region of Cameroon
The presence of ochratoxin A (OTA) in cocoa beans is a major health concern, due to its deleterious effects on humans and animals. During the traditional processing and storage of cocoa, fungi contamination occurs. Many...