Isolation and Identification of Potential Probiotics Bacteria from the Gut of Oreochromis niloticus and Clarias gariepinus in Uganda
Journal Title: Microbiology Research Journal International - Year 2016, Vol 17, Issue 5
Abstract
Bacterial infections remain a hindrance to aquaculture expansion globally. Increased fish mortality and poor performance resulting from ill health has forced farmers to resort to the use of antibiotics globally. However, prolonged use of these drugs in aquaculture is becoming restrained as pathogens develop resistance to drugs and unpredicted long term effect on public health. Alternative approaches to control disease are proposed of which probiotics have come forward. Therefore, the purpose of this study was to identify potential probiotic bacteria in the guts of fish from different sampling sites around Kampala. Fish were obtained from selected cages, ponds, tanks and hatcheries around Kampala, including different parts of Lake Victoria. The fish were gutted and the guts aseptically swabbed with subsequent culture on both general purpose and selective media. The identification of various isolates was based on gram staining and biochemical tests. Probiotic screening was done using the agar spot method. Results revealed complete growth across all samples. The total microbial load was highest in the samples from the lake (1204.8±12.7 × 105cfu/g). Out of the three probiotic genera isolated, only Lactobacillus spp (LB) and Lactococcus spp (LC) showed antibacterial activity against selected pathogenic bacteria. The activity of LB was significantly (p< 0.0001) higher against Streptococcus spp (17.0±0.2 mm) as compared to Proteus at 9±0.02 mm and Pseudomonas (7.5±0.2 mm). Lactobacillus spp did not show any antimicrobial activity against Staphylococcus aureus. For Lactococcus spp, probiotic activity was only detected against Proteus spp (5.5±0.2 mm). Although our study shows that Lactobacillus spp and Lactococcus spp possess probiotic activity against a number of pathogenic bacteria, characterization of these isolates is paramount before further manipulation.
Authors and Affiliations
Charles Drago Kato, Mark Benjamin Mugaanyi, Samuel Majalija, Andrew Tamale, Nathan Lubowa Musisi, Asuman Sengooba
Keywords
Related Articles
- EP ID EP350735
- DOI 10.9734/BMRJ/2016/29721
- Views 75
- Downloads 0
Phytochemical Study and Antibacterial Properties of the Leaf Extracts of Swartzia madagascariensis Desv (Fabaceae)
This study evaluated the phytochemical and antibacterial properties of the hexane, ethyl acetate and ethanol leaf extracts of Swartzia madagascariensis Desv (Fabaceae). Ethno-medicinally, the leaves are used in the treat...
Potential Spread of Pathogens by Consumption of Locally Produced Zobo and Soya Milk Drinks in Awka Metropolis, Nigeria
Aims: To determine the presence of resistant pathogens in locally produced drinks consumed in Akwa, Nigeria. Study Design: To determine type of bacteria contaminants, level of contamination from and presence of resistan...
Isolation and Identification of Some Enterobacteria from Retailed Convenience Foods
Meat pie and Fish roll samples from five different local government areas (LGAs) in Ibadan, Nigeria were analysed microbiologically for presence of Enterobacteriaceae and other common food borne pathogens. Sampling was d...
Aflatoxins Investigation and Mycobiota of Selected Marketed Smoked - Dried Fish Samples in Ado-Ekiti, Nigeria and Their Environmental Health Implications
Aims: This study was carried out to assess the mycobiota and aflatoxins contamination in selected common smoked-dried fish samples sold at Ojo Oba market in Ado Ekiti, Nigeria and their environmental health implications....
Evaluation of DNA Based Techniques for the Diagnosis of Human Vaginal Trichomoniasis in North Indian Population
Introduction: Human trichomoniasis due to Trichomonas vaginalis is a curable sexually transmitted infection. It may lead to symptomatic vaginitis or asymptomatic carrier state. The symptoms and signs mimic other patholog...