Isolation and in silico characterization of cinnamate 4-hydroxylase (C4H) gene controlling the early stage of phenylpropanoid biosynthetic pathway in Kelampayan (Neolamarckia cadamba, Rubiaceae) developing xylem tissues
Journal Title: Asian Journal of Agriculture and Biology - Year 2018, Vol 6, Issue 2
Abstract
Cinnamate 4-hydroxylase (C4H) is one of the enzymes involved at the starting point of the phenylpropanoid and lignin biosynthesis pathway. It involves in the hydroxylation of cinnamate to 4-coumarate. In this paper, we isolated and in silico characterized the complete sequence of cinnamate 4-hydroxylase (C4H) gene from Neolamarckia cadamba in Malaysia. The C4H singletons obtained from the NcdbEST were used to predict the hypothetical full-length of NcC4H through the contig mapping approach. RT-PCR was used to amplify the full-length C4H cDNA clone and subsequently the PCR amplicons were sequenced and analysed. The NcC4H cDNA was 1,651 bp long with a 505 amino acid sequence, a 18 bp 5’-UTR and a 115 bp 3’-UTR. The predicted NcC4H protein contains P450-featured motifs. These include the heme-binding domain, a threonine-containing binding pocket motif and the proline-rich region. Peptide sequence comparison and phylogenetic analyses revealed that NcC4H was clustered with class I C4H instead of class II C4H, which is preferentially involved in phenylpropanoid and lignin biosynthesis pathway. This full-length NcC4H cDNA can be used for developing genetic marker to identify economic trait loci (ETL) for wood quality traits via genomics-assisted selection (GAS) or candidate gene mapping approach.
Authors and Affiliations
Wei Seng – Ho
Keywords
Related Articles
- EP ID EP604168
- DOI -
- Views 93
- Downloads 0
Screening of breeding lines of Brassica napus L. tolerant to grain shattering
Shattering causes huge losses to Brassica even if harvesting is done mechanically. To study the shattering tolerance in different rapeseeds lines, a study was conducted in split plot design at experimental area of Barani...
Determination of the critical period for weed control of sweet corn under tropical organic farming system
An understanding of the critical period of the crop for weed control (CPWC) is needed before making a decision on weed management. A field experiment to study a CPWC of sweet corn was carried out at the highland of Beng...
The characteristics of fungi contaminating chicken feed in Tegal, Bogor, West Java
Fungi can cause contamination in animal feed. Contamination of the feed may result in damage to feed quality and decrease the health of livestock. The aim of this study was to isolate and identify fungi strains in cage-...
Spectroscopic and morphological characteristics of genus Jatropha (Euphorbiaceae) and genus Jojoba (Simmondsiaceae)
Using second generation of biofuel that does not affect food crop production is an issue of global concern. Remote sensing (RS) proved to be efficient technique for inventory and monitoring the spatial distribution of b...
Physiological Studies on Colletotrichum Gloeosporiodes Associated With Wither Tip Disease of Citrus and Its Chemical Control
Citrus is the second largest fruit produced in the world and Pakistan is among the 12 large producers of the citrus fruit. It is grown in tropical and subtropical climate all over the world. Besides its high economical &...